Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   NTHIC486_RS03110 Genome accession   NZ_CP007471
Coordinates   613729..614268 (-) Length   179 a.a.
NCBI ID   WP_044345365.1    Uniprot ID   -
Organism   Haemophilus influenzae strain C486     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 608729..619268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHIC486_RS03080 (NTHIC486_00616) fdx 609134..609475 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  NTHIC486_RS03085 (NTHIC486_00617) iscX 609475..609669 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  NTHIC486_RS03090 (NTHIC486_00618) - 609718..610332 (-) 615 WP_044345361.1 YfgM family protein -
  NTHIC486_RS03095 (NTHIC486_00619) hisS 610350..611621 (-) 1272 WP_044345363.1 histidine--tRNA ligase -
  NTHIC486_RS03100 (NTHIC486_00620) ispG 611631..612737 (-) 1107 WP_005686814.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NTHIC486_RS03105 (NTHIC486_00621) - 612746..613657 (-) 912 WP_005686812.1 helix-turn-helix domain-containing protein -
  NTHIC486_RS03110 (NTHIC486_00622) pilF/pilF2 613729..614268 (-) 540 WP_044345365.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NTHIC486_RS03115 (NTHIC486_00623) - 614369..615520 (-) 1152 WP_044345367.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NTHIC486_RS03120 (NTHIC486_00624) - 615767..616645 (+) 879 WP_005656360.1 D-alanyl-D-alanine carboxypeptidase family protein -
  NTHIC486_RS03125 (NTHIC486_00625) - 617010..617891 (+) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -
  NTHIC486_RS03130 (NTHIC486_00626) - 617891..618811 (+) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20652.47 Da        Isoelectric Point: 9.2047

>NTDB_id=119741 NTHIC486_RS03110 WP_044345365.1 613729..614268(-) (pilF/pilF2) [Haemophilus influenzae strain C486]
MQTISKQLSAVIFPFIFSACVSQSVSNLDHQAAAKVRVELALSYLQQNNPQLAKINLDKALLHDKNYYLVHSALAHYYQQ
QGQIKNAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQTDTFENIALCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=119741 NTHIC486_RS03110 WP_044345365.1 613729..614268(-) (pilF/pilF2) [Haemophilus influenzae strain C486]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGTATCTAA
CTTAGATCATCAAGCTGCCGCCAAAGTGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCTACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAAAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCTCAACAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAACAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

93.296

100

0.933
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment