Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   NTHI477_RS06975 Genome accession   NZ_CP007470
Coordinates   1450990..1451532 (+) Length   180 a.a.
NCBI ID   WP_044332524.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 477     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1445990..1456532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHI477_RS06955 (NTHI477_01404) - 1446871..1447752 (-) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -
  NTHI477_RS06960 (NTHI477_01405) - 1448058..1448522 (+) 465 WP_044332514.1 hypothetical protein -
  NTHI477_RS06965 (NTHI477_01406) - 1448614..1449492 (-) 879 WP_044332518.1 D-alanyl-D-alanine carboxypeptidase family protein -
  NTHI477_RS06970 (NTHI477_01407) - 1449738..1450889 (+) 1152 WP_044332521.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NTHI477_RS06975 (NTHI477_01408) pilF/pilF2 1450990..1451532 (+) 543 WP_044332524.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NTHI477_RS06980 (NTHI477_01409) - 1451604..1452515 (+) 912 WP_044332526.1 RodZ domain-containing protein -
  NTHI477_RS06985 (NTHI477_01410) ispG 1452524..1453630 (+) 1107 WP_044332529.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NTHI477_RS06990 (NTHI477_01411) hisS 1453640..1454911 (+) 1272 WP_038440737.1 histidine--tRNA ligase -
  NTHI477_RS06995 (NTHI477_01412) - 1454929..1455543 (+) 615 WP_011272010.1 YfgM family protein -
  NTHI477_RS07000 (NTHI477_01413) iscX 1455594..1455788 (-) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  NTHI477_RS07005 (NTHI477_01414) fdx 1455788..1456129 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20806.51 Da        Isoelectric Point: 8.9410

>NTDB_id=119705 NTHI477_RS06975 WP_044332524.1 1450990..1451532(+) (pilF/pilF2) [Haemophilus influenzae strain 477]
MQTISKQLSAVIFPFIFSACVSQSTIQDFNQQSAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQ
QQGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMNIYQQ
TLEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=119705 NTHI477_RS06975 WP_044332524.1 1450990..1451532(+) (pilF/pilF2) [Haemophilus influenzae strain 477]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCCCAATCAACTATTCA
AGACTTCAATCAACAATCAGCAGCGAAAGCTCGCGTAGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGG
CTAAAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAA
CAACAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAA
TAATTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCCCTTAATTCGCCGA
ATTATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGAATATTTATCAGCAA
ACATTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

93.333

100

0.933
  pilF Glaesserella parasuis strain SC1401

48.045

99.444

0.478

Multiple sequence alignment