Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CD58_RS18760 Genome accession   NZ_CP007410
Coordinates   4425513..4426628 (-) Length   371 a.a.
NCBI ID   WP_025214532.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain DF41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4420513..4431628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CD58_RS18745 (CD58_19500) - 4421055..4422446 (+) 1392 WP_025214529.1 GntP family permease -
  CD58_RS18750 (CD58_19505) - 4422454..4423230 (+) 777 WP_025214530.1 3-hydroxybutyrate dehydrogenase -
  CD58_RS18755 (CD58_19510) - 4423406..4425361 (+) 1956 WP_025214531.1 acetoacetate--CoA ligase -
  CD58_RS18760 (CD58_19515) pilU 4425513..4426628 (-) 1116 WP_025214532.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CD58_RS18765 (CD58_19520) - 4426761..4429298 (+) 2538 WP_025214533.1 PAS domain-containing protein -
  CD58_RS18770 (CD58_19525) - 4429344..4429619 (+) 276 WP_019690952.1 peptidylprolyl isomerase -
  CD58_RS18775 (CD58_19530) - 4429772..4430695 (-) 924 WP_025214534.1 sugar kinase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41188.10 Da        Isoelectric Point: 6.0667

>NTDB_id=119265 CD58_RS18760 WP_025214532.1 4425513..4426628(-) (pilU) [Pseudomonas brassicacearum strain DF41]
MEIDPLLRILASQDGSDLYMSTGAPPCARFEGVLKPLGNQAFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISMAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQSFDQAL
FNLVVEGAIDEEEALKNADSANNLRLRLKLHAESGAAPPPDPAAGEWGLVD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=119265 CD58_RS18760 WP_025214532.1 4425513..4426628(-) (pilU) [Pseudomonas brassicacearum strain DF41]
ATGGAAATCGATCCCTTGTTGCGAATCCTGGCGAGCCAGGATGGCTCCGACCTCTACATGTCCACCGGCGCGCCACCGTG
CGCGCGTTTCGAGGGCGTGCTCAAGCCCTTGGGTAACCAGGCATTCAAGGTCGGCGACATTGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAACTGGAAATGAACCTGGCGATCTCCATGGCCGGTGTCGGGCGG
TTCCGGGTGAATATTTTCAAGCAGCGCAACGACGTGTCCATGGTGATACGCAACGTCAAGCTGGACATTCCGCGCTTCGA
AGACCTGAAGCTGCCGCGGGTATTGCTGGACACCATTATGCAGAAACAGGGGCTGATGCTGTTCGTTGGCGCGACAGGCT
CGGGCAAGTCGACGTCCCTGGCGGCACTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCAATCATCAATCAGCGGGAAGTCGGTGTCGACACCCGCAGTTTTCATGC
CGCCTTGAAGAACACCTTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAGATCCGTGATCGCGAAACCATGGAACACG
CGCTGTCCTTCGCCGACACCGGGCACCTGGTGATTTCCACGCTGCATGCCCATAACGCCAACCAGGCCCTGGATCGGGTG
ATCAACTTCTTCCCGGAAGAGCGCCGGCCGCAGTTGCTCAATGACCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
CCTGGTGCGCACCCGTACCGGCCAGCGCCGGGCGGCGGTGGAGGTCATGCTGGGCTCGCCCACGGTGGCCGATTTGATCC
GGCGCAACGAGCTCGGCGAACTCAAGGGGATTATGGAAAAGTCAGAGGAGCTGGGCATGCAAAGCTTCGACCAGGCCTTG
TTCAACCTGGTGGTAGAGGGGGCGATCGACGAGGAAGAAGCCTTGAAAAACGCCGACTCGGCGAACAACTTGCGCTTGCG
TTTGAAGTTGCATGCCGAGTCGGGCGCGGCGCCTCCACCTGACCCGGCGGCGGGGGAGTGGGGGTTGGTGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.056

95.687

0.536
  pilU Vibrio cholerae strain A1552

52.571

94.34

0.496
  pilU Acinetobacter baylyi ADP1

51.261

96.226

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.005

100

0.38
  pilT Pseudomonas stutzeri DSM 10701

38.953

92.722

0.361

Multiple sequence alignment