Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RG53_RS23635 Genome accession   NZ_CP010178
Coordinates   4590980..4591420 (-) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain H15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4585980..4596420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG53_RS23615 (RG53_22550) coaE 4586470..4587090 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  RG53_RS26175 - 4587115..4587159 (+) 45 WP_120795372.1 protein YacM -
  RG53_RS23620 (RG53_22555) guaC 4587315..4588358 (+) 1044 WP_073527968.1 GMP reductase -
  RG53_RS23625 (RG53_22560) hofC 4588393..4589595 (-) 1203 WP_000157229.1 protein transport protein HofC -
  RG53_RS23630 (RG53_22565) pilB 4589585..4590970 (-) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  RG53_RS23635 (RG53_22570) pilA 4590980..4591420 (-) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  RG53_RS23645 (RG53_22580) nadC 4591623..4592516 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG53_RS23650 (RG53_22585) ampD 4592604..4593155 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG53_RS23655 (RG53_22590) ampE 4593152..4594006 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  RG53_RS23660 (RG53_22595) aroP 4594049..4595422 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=117351 RG53_RS23635 WP_000360902.1 4590980..4591420(-) (pilA) [Escherichia coli strain H15]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=117351 RG53_RS23635 WP_000360902.1 4590980..4591420(-) (pilA) [Escherichia coli strain H15]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377

Multiple sequence alignment