Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACMFWR_RS03210 Genome accession   NZ_OZ221287
Coordinates   760587..762092 (-) Length   501 a.a.
NCBI ID   WP_413193593.1    Uniprot ID   -
Organism   Pararobbsia alpina isolate ABIP432     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 755587..767092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMFWR_RS03185 (CAB432_A0723) - 756367..756993 (-) 627 WP_413193588.1 LON peptidase substrate-binding domain-containing protein -
  ACMFWR_RS03190 (CAB432_A0724) rapZ 757021..757896 (-) 876 WP_413193589.1 RNase adapter RapZ -
  ACMFWR_RS03195 (CAB432_A0725) hprK 758065..759033 (-) 969 WP_413193590.1 HPr(Ser) kinase/phosphatase -
  ACMFWR_RS03200 (CAB432_A0726) ptsN 759156..759611 (-) 456 WP_413193591.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACMFWR_RS03205 (CAB432_A0727) hpf 759980..760351 (-) 372 WP_413193592.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACMFWR_RS03210 (CAB432_A0728) rpoN/rpoN1 760587..762092 (-) 1506 WP_413193593.1 RNA polymerase factor sigma-54 Machinery gene
  ACMFWR_RS03215 (CAB432_A0729) lptB 762535..763317 (-) 783 WP_413193594.1 LPS export ABC transporter ATP-binding protein -
  ACMFWR_RS03220 (CAB432_A0730) lptA 763464..764078 (-) 615 Protein_638 lipopolysaccharide transport periplasmic protein LptA -
  ACMFWR_RS03225 (CAB432_A0731) lptC 764150..764740 (-) 591 WP_413193595.1 LPS export ABC transporter periplasmic protein LptC -
  ACMFWR_RS03230 (CAB432_A0732) - 764755..765243 (-) 489 WP_413194762.1 KdsC family phosphatase -
  ACMFWR_RS03235 (CAB432_A0733) - 765333..766319 (-) 987 WP_413193596.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55041.96 Da        Isoelectric Point: 4.6984

>NTDB_id=1170511 ACMFWR_RS03210 WP_413193593.1 760587..762092(-) (rpoN/rpoN1) [Pararobbsia alpina isolate ABIP432]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAVQQNPLLENDEDWLSSPLRVAADGSVLASQQSAPPTAPEA
PTSSIPSGGNGESEETHERNGLDDFSNGSGDSDSPWSLDDYSRPGSAADDDDLPPMQLQESGTSLRDHLSVQLRLTSASP
RDRALVTFLIESLDDDGYLSMSLDEVVADLPEELEIDTDEAGAALALLQSFDPPGVGARSASECLKIQLLRMDDSSIREL
AIDIVSQHLELLAARDFTRLRKQLKVGDDDLRDAHTLIRSLEPFPGAAYGKAEADYVVPDVIVRKTKDGWIADLNSDIVP
RLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVDRQKNFFVHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEEPKTPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1170511 ACMFWR_RS03210 WP_413193593.1 760587..762092(-) (rpoN/rpoN1) [Pararobbsia alpina isolate ABIP432]
ATGAAAGCGAGCCTCCAACTTCGTCTCTCGCAGCATCTGGCCTTGACCCCGCAATTGCAGCAGTCCATCCGGCTGCTGCA
ATTGTCGACGCTCGAACTCCAGCAGGAGGTGGCGACCGCGGTCCAGCAGAACCCGCTGCTCGAGAACGACGAGGACTGGC
TGAGCAGCCCGCTGCGCGTGGCGGCCGATGGTTCGGTGCTCGCGTCGCAGCAAAGCGCGCCGCCGACGGCCCCCGAGGCC
CCGACCAGTTCGATCCCTTCCGGCGGCAACGGCGAAAGCGAAGAAACGCACGAGCGAAACGGGCTCGACGATTTCAGCAA
TGGTTCGGGAGACAGTGACTCGCCCTGGAGCCTGGACGACTATTCACGCCCGGGCAGTGCGGCCGACGACGACGATTTGC
CTCCGATGCAGCTTCAGGAGTCCGGCACCAGCCTGCGCGATCACCTGAGCGTCCAGCTGCGCCTGACGAGCGCCAGCCCG
CGTGACCGTGCGCTCGTGACCTTCCTGATCGAATCGCTCGACGACGATGGCTATCTGTCGATGTCGCTCGACGAGGTCGT
TGCGGACCTGCCCGAAGAACTCGAGATCGATACCGACGAAGCGGGCGCCGCGCTGGCGCTGCTTCAGAGTTTCGATCCGC
CGGGAGTCGGCGCGCGCTCGGCGTCGGAATGTCTGAAGATCCAGTTGCTGCGCATGGACGACTCGTCGATCCGCGAACTG
GCGATCGATATCGTGTCGCAGCATCTCGAACTGCTTGCGGCGCGCGACTTCACGCGCCTGCGCAAGCAGCTCAAGGTCGG
CGACGACGACCTGCGCGACGCCCACACGCTGATCCGCTCGCTCGAGCCCTTCCCGGGCGCGGCGTACGGCAAGGCTGAAG
CCGACTATGTGGTGCCCGACGTGATCGTGCGCAAGACCAAGGACGGCTGGATCGCCGACCTCAACTCGGACATCGTGCCG
AGGCTGCGCATCAATCACCTGTATGCCAACATCCTGCGCAACAATCGCGGCGATCCGGGCAGTGGCTCATTGCGCCAGCA
ACTCCAGGAAGCCCGCTGGCTGATAAAAAACATCCAGCAACGCTTCGAAACGATATTGAGAGTTGCGCAGGCGATCGTCG
ATCGGCAGAAGAATTTTTTTGTTCACGGCGAAATCGCCATGCGCCCCTTGGTCTTGCGTGAGATTGCTGATACGCTGGGG
CTACACGAATCAACGGTTTCCCGCGTAACGACCGGCAAGTACATGCTGACGCCCTTCGGGACGCTCGAATTCAAGTACTT
CTTTGGTTCGCACGTGTCGACCGACACCGGCGGGGCAGCATCCTCGACGGCGATCCGCGCCCTCATCAAGCAACTGATAG
GAGCCGAAGAACCGAAGACACCTCTTTCAGACAGCCGCATCGCTGAACTGCTGGCTGAACAGGGTTTCGTGGTCGCCCGC
CGCACCGTGGCCAAATATCGAGAAGCCCTGAAGATCCCAGCCGTCAACCTGCGCAAGTCCCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.317

100

0.689

Multiple sequence alignment