Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   ACLV7W_RS01285 Genome accession   NZ_OZ217346
Coordinates   257398..258222 (+) Length   274 a.a.
NCBI ID   WP_078237947.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis D22     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 252398..263222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7W_RS01250 (SMID22_02510) rsmD 252453..252992 (+) 540 WP_261077988.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACLV7W_RS01255 (SMID22_02520) coaD 252982..253470 (+) 489 WP_153209764.1 pantetheine-phosphate adenylyltransferase -
  ACLV7W_RS01260 (SMID22_02530) sepM 253454..254491 (+) 1038 WP_153209763.1 SepM family pheromone-processing serine protease Regulator
  ACLV7W_RS01265 (SMID22_02540) - 254548..254778 (+) 231 WP_153209762.1 DUF1146 family protein -
  ACLV7W_RS01270 (SMID22_02550) - 254840..255868 (+) 1029 WP_411865285.1 acyltransferase family protein -
  ACLV7W_RS01275 (SMID22_02560) murA 255895..257178 (+) 1284 WP_045612767.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  ACLV7W_RS01280 (SMID22_02570) - 257171..257359 (+) 189 WP_004241151.1 DNA-directed RNA polymerase subunit beta -
  ACLV7W_RS01285 (SMID22_02580) endA 257398..258222 (+) 825 WP_078237947.1 DNA-entry nuclease EndA Machinery gene
  ACLV7W_RS01290 (SMID22_02590) - 258490..259821 (+) 1332 WP_000390027.1 hemolysin family protein -
  ACLV7W_RS01300 (SMID22_02610) amiA 260247..262226 (+) 1980 WP_411865286.1 peptide ABC transporter substrate-binding protein Regulator

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29940.51 Da        Isoelectric Point: 9.7812

>NTDB_id=1170415 ACLV7W_RS01285 WP_078237947.1 257398..258222(+) (endA) [Streptococcus mitis isolate S. mitis D22]
MDKKTRQTLIGLLVLLLLSAGSYYIKQMQSTSNSPKTKLSQKKQASEAPSQELAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESMVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=1170415 ACLV7W_RS01285 WP_078237947.1 257398..258222(+) (endA) [Streptococcus mitis isolate S. mitis D22]
ATGGATAAAAAAACAAGACAGACACTGATCGGACTGCTAGTGTTATTACTCTTATCTGCGGGAAGCTATTATATCAAGCA
GATGCAGTCGACATCTAATAGTCCTAAAACCAAACTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGAGTTGG
CAGAAAGTGTCTTAACAGATGCAGTCAAAAGCCAAATAAAAGGGAGTCTTGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAACCTTACGCTGATAATAAAACAAAGACAGTAGGCAAGGA
AACAGTGCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCTACTCGCCAGTACAAGAATCGTGAAGAAACTGGGAATGGTT
CGACTTCATGGACTCCTCCAGGCTGGCATCAGGTTAAGAATCTAAAGGGGTCTTATACCCATGCGGTCGATAGAGGTCAC
TTGTTAGGCTATGCCTTAATCGGTGGCTTGGATGGTTTTGATGCCTCGACAAGCAATCCTAAAAACATTGCTGTTCAAAC
AGCCTGGGCAAATCAGGCTCAAGCCGAGGATTCGACTGGTCAAAACTACTATGAAAGCATGGTACGTAAGGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACTCTTTATTATGCTTCAAACGAGGATTTAGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCTTCAGACGGAGAATTGGAATTTAATGTCCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACGGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

96.35

100

0.964
  endA Streptococcus pneumoniae D39

96.35

100

0.964
  endA Streptococcus pneumoniae R6

96.35

100

0.964
  endA Streptococcus pneumoniae TIGR4

96.35

100

0.964

Multiple sequence alignment