Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACLV7W_RS00135 Genome accession   NZ_OZ217346
Coordinates   24064..25425 (+) Length   453 a.a.
NCBI ID   WP_077804517.1    Uniprot ID   A0A2N6PNQ5
Organism   Streptococcus mitis isolate S. mitis D22     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 19064..30425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7W_RS00105 (SMID22_00180) comW 20483..20719 (+) 237 WP_194251552.1 sigma(X)-activator ComW Regulator
  ACLV7W_RS00110 (SMID22_00190) - 20951..22237 (+) 1287 WP_153210200.1 adenylosuccinate synthase -
  ACLV7W_RS00115 (SMID22_00210) tadA 22437..22904 (+) 468 WP_153210198.1 tRNA adenosine(34) deaminase TadA -
  ACLV7W_RS00125 (SMID22_00220) - 23090..23533 (+) 444 WP_000701987.1 dUTP diphosphatase -
  ACLV7W_RS00130 (SMID22_00230) - 23493..24050 (+) 558 WP_411865215.1 histidine phosphatase family protein -
  ACLV7W_RS00135 (SMID22_00240) radA 24064..25425 (+) 1362 WP_077804517.1 DNA repair protein RadA Machinery gene
  ACLV7W_RS00140 (SMID22_00250) - 25498..25995 (+) 498 WP_261241057.1 beta-class carbonic anhydrase -
  ACLV7W_RS00145 (SMID22_00260) - 26020..26835 (+) 816 WP_411865216.1 PrsW family intramembrane metalloprotease -
  ACLV7W_RS00150 (SMID22_00270) - 26981..27949 (+) 969 WP_033682853.1 ribose-phosphate diphosphokinase -
  ACLV7W_RS00155 - 28041..29392 (+) 1352 WP_411865217.1 IS3 family transposase -
  ACLV7W_RS00160 (SMID22_00300) - 29503..29940 (+) 438 WP_001863803.1 CoA-binding protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49416.91 Da        Isoelectric Point: 6.5137

>NTDB_id=1170410 ACLV7W_RS00135 WP_077804517.1 24064..25425(+) (radA) [Streptococcus mitis isolate S. mitis D22]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1170410 ACLV7W_RS00135 WP_077804517.1 24064..25425(+) (radA) [Streptococcus mitis isolate S. mitis D22]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGATCTTGGTCTTCTTTTGTAGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAACCCATGAAACTGGCTGAGGTGACTTCTATAAACGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTA
CTTGGAGGAGGAGTAGTACCGGGCAGTCTCGTCCTCATAGGTGGGGATCCTGGGATTGGGAAATCAACCCTTCTCTTACA
AGTCTCAACCCAGTTGTCTCAAGTGGGGACTGTTCTCTACGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAATTACGTG
CAGAGCGTTTGGGAGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGAGCTGAGGTGGAG
CGCATCCAGCCAGACTTCCTCATTATTGACTCAATTCAAACCATTATGTCTCCTGAGATTTCAGGGGTGCAGGGATCTGT
TTCTCAAGTGCGTGAGGTGACGGCTGAGCTTATGCAACTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAGGAAGGAACCTTGGCTGGTCCTCGTATGCTAGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGC
CACCATACCTTCCGTATTTTAAGAGCGGTCAAAAACCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGATTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCGTCAATCG
TTGTGACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCCTTGGTGACACCGACCATGTTTGGAAATGCTAAG
CGTACGACGACAGGACTTGATTTTAATCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGTCTTCTTTTGCA
AAATCAGGATGCTTATCTTAAATCTGCTGGCGGTGTCAAATTAGATGAACCTGCCATTGACTTGGCCGTTGCAGTTGCCA
TTGCTTCGAGTTATAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTAGGCGAACTGGGCTTGACAGGAGAAATTCGG
CGTGTGAATCGTATCGAGCAACGCATCAATGAAGCTGCTAAGCTTGGATTTACAAAAATCTATGTACCGAAAAATTCCTT
GACAGGAATCACCCCGCCCAAGGAAATTCAGGTCATTGGAGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N6PNQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

99.779

100

0.998
  radA Streptococcus mitis SK321

99.779

100

0.998
  radA Streptococcus pneumoniae Rx1

99.779

100

0.998
  radA Streptococcus pneumoniae D39

99.779

100

0.998
  radA Streptococcus pneumoniae R6

99.779

100

0.998
  radA Streptococcus pneumoniae TIGR4

99.779

100

0.998
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment