Detailed information NTG1170355

Bioinformatically predicted

Overview

• Name: amiF
• Type: Regulator
• Locus tag: ACLV7D_RS02020
• Genome accession: NZ_OZ217345
• Coordinates: 385902..386828 (+)
• Length: 308 a.a.
• NCBI ID: WP_001291301.1
• Uniprot ID: A0A1X1JQM1
• Organism: Streptococcus mitis isolate S. mitis F22
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 380902..391828

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV7D_RS02005 (SMIF22_03850)	amiC	382393..383889 (+)	1497	WP_112443790.1	ABC transporter permease	Regulator
ACLV7D_RS02010 (SMIF22_03860)	amiD	383889..384815 (+)	927	WP_000103703.1	oligopeptide ABC transporter permease OppC	Regulator
ACLV7D_RS02015 (SMIF22_03870)	amiE	384824..385891 (+)	1068	WP_218764302.1	ABC transporter ATP-binding protein	Regulator
ACLV7D_RS02020 (SMIF22_03880)	amiF	385902..386828 (+)	927	WP_001291301.1	ATP-binding cassette domain-containing protein	Regulator
ACLV7D_RS02025 (SMIF22_03890)	-	387281..388258 (+)	978	WP_218764303.1	ABC transporter substrate binding protein	-
ACLV7D_RS02030 (SMIF22_03900)	-	388270..389163 (+)	894	WP_173233257.1	ABC transporter permease	-
ACLV7D_RS02035 (SMIF22_03910)	-	389176..389979 (+)	804	WP_000157661.1	ABC transporter ATP-binding protein	-
ACLV7D_RS02040 (SMIF22_03920)	-	390000..390149 (+)	150	Protein_347	alpha,alpha-phosphotrehalase	-
ACLV7D_RS02045 (SMIF22_03930)	-	390290..390538 (+)	249	WP_000364990.1	YneF family protein	-
ACLV7D_RS02050 (SMIF22_03940)	racE	390737..391531 (+)	795	WP_173274771.1	glutamate racemase	-

Sequence

Protein

• Length: 308 a.a.
• Molecular weight: 34791.87 Da
• Isoelectric Point: 6.7096

>NTDB_id=1170355 ACLV7D_RS02020 WP_001291301.1 385902..386828(+) (amiF) [Streptococcus mitis isolate S. mitis F22]
MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGESGSGKTTIGRAIIGLNDTSNGDIIFEGQKINGKKSRE
QAAELIRRIQMIFQDPAASLNERATVDYIISEGLYNHHLFKDEEERKEKVKNIIREVGLLAEHLTRYPHEFSGGQRQRIG
IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFISDRIAVIYKGVIVEVAETEELFN
NPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHDYETDKPSMVEIRPGHYVWANQAELARYQKGLN

Nucleotide

• Length: 927 bp

>NTDB_id=1170355 ACLV7D_RS02020 WP_001291301.1 385902..386828(+) (amiF) [Streptococcus mitis isolate S. mitis F22]
ATGTCTGAAAAATTAGTAGAAATCAAAGATTTAGAAATTTCCTTCGGTGAAGGAAGTAAGAAGTTTGTCGCAGTTAAAAA
TGCTAACTTCTTTATCAACAAGGGAGAAACGTTCTCGCTTGTAGGTGAGTCAGGAAGTGGGAAAACAACTATTGGTCGTG
CTATCATCGGTCTAAATGATACAAGTAATGGAGATATCATTTTTGAGGGTCAAAAGATTAATGGTAAGAAATCGCGTGAA
CAAGCTGCGGAATTGATTCGTCGTATCCAGATGATTTTCCAAGACCCTGCGGCAAGTTTGAATGAACGTGCGACTGTTGA
TTATATTATTTCTGAAGGTCTTTACAATCACCATTTGTTTAAGGATGAAGAAGAACGTAAAGAGAAAGTTAAAAATATTA
TCCGTGAAGTGGGGCTTCTTGCTGAGCATTTGACTCGTTACCCTCATGAATTCTCAGGTGGTCAACGTCAACGTATCGGT
ATTGCCCGTGCCTTGGTCATGCAACCAGATTTTGTCATTGCGGATGAACCCATTTCAGCCTTGGACGTTTCTGTGCGTGC
CCAAGTCTTGAACTTGCTCAAAAAATTCCAAAAAGAACTTGGCTTGACCTATCTCTTTATCGCCCATGACTTGTCGGTTG
TTCGCTTTATTTCAGATCGTATCGCAGTTATTTACAAGGGTGTTATTGTAGAGGTTGCAGAAACAGAAGAATTGTTTAAC
AATCCAATACACCCATATACTCAAGCCTTGCTTTCAGCGGTACCGATTCCAGATCCAATCTTGGAACGTAAGAAGGTCTT
GAAGGTTTACGACCCAAGTCAACACGACTATGAGACTGATAAGCCATCTATGGTAGAAATCCGTCCAGGTCACTATGTTT
GGGCGAACCAAGCCGAATTGGCACGTTATCAAAAAGGACTAAACTAA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (25-178)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (230-262)

3D structure

• AlphaFold DB: A0A1X1JQM1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiF	Streptococcus thermophilus LMG 18311	85.902	99.026	0.851
amiF	Streptococcus salivarius strain HSISS4	85.902	99.026	0.851
amiF	Streptococcus thermophilus LMD-9	85.574	99.026	0.847