Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACLV7D_RS00145 Genome accession   NZ_OZ217345
Coordinates   25883..27244 (+) Length   453 a.a.
NCBI ID   WP_411865968.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis F22     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20883..32244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7D_RS00110 - 21838..22070 (-) 233 Protein_15 transposase -
  ACLV7D_RS00115 (SMIF22_00190) comW 22301..22537 (+) 237 WP_173224583.1 sigma(X)-activator ComW Regulator
  ACLV7D_RS00120 (SMIF22_00200) - 22768..24054 (+) 1287 WP_173251393.1 adenylosuccinate synthase -
  ACLV7D_RS00125 (SMIF22_00220) tadA 24255..24722 (+) 468 WP_164227012.1 tRNA adenosine(34) deaminase TadA -
  ACLV7D_RS00135 (SMIF22_00240) - 24909..25352 (+) 444 WP_000701983.1 dUTP diphosphatase -
  ACLV7D_RS00140 (SMIF22_00250) - 25303..25869 (+) 567 WP_218764136.1 histidine phosphatase family protein -
  ACLV7D_RS00145 (SMIF22_00260) radA 25883..27244 (+) 1362 WP_411865968.1 DNA repair protein RadA Machinery gene
  ACLV7D_RS00150 (SMIF22_00270) - 27317..27814 (+) 498 WP_218764137.1 beta-class carbonic anhydrase -
  ACLV7D_RS00155 (SMIF22_00280) - 27839..28654 (+) 816 WP_218764138.1 PrsW family intramembrane metalloprotease -
  ACLV7D_RS00160 (SMIF22_00290) - 28799..29767 (+) 969 WP_033682853.1 ribose-phosphate diphosphokinase -
  ACLV7D_RS00165 (SMIF22_00300) - 30037..30984 (+) 948 WP_218764139.1 Rpn family recombination-promoting nuclease/putative transposase -
  ACLV7D_RS00170 - 30968..31156 (+) 189 WP_218764140.1 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49416.91 Da        Isoelectric Point: 6.5133

>NTDB_id=1170328 ACLV7D_RS00145 WP_411865968.1 25883..27244(+) (radA) [Streptococcus mitis isolate S. mitis F22]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSIRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1170328 ACLV7D_RS00145 WP_411865968.1 25883..27244(+) (radA) [Streptococcus mitis isolate S. mitis F22]
ATCGCAAAGAAAAAAGCAACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGTCCTAACTG
TGGGTCTTGGTCTTCTTTTGTAGAAGAGGTTGAGGTTGCCGAGGTCAAAAATGCGCGTGTGTCTTTAACAGGTGAGAAAA
CCAAGCCCATGAAACTGGCTGAGGTGACTTCTATCAATGTCAATCGAACCAAGACGGAGATGGACGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTAGTCCTCATTGGTGGGGATCCTGGGATTGGGAAATCAACCCTTCTCTTACA
AGTCTCAACCCAGCTGTCTCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAAATCAAGCTACGAG
CAGAGCGCTTGGGTGATATTGATAGTGAGTTTTACCTCTATGCAGAAACAAATATGCAGAGTATTCGCGCAGAGGTAGAG
AGAATCCAGCCAGACTTTCTCATCATCGACTCTATCCAGACCATTATGTCTCCTGAGATTTCAGGGGTGCAAGGATCTGT
TTCTCAGGTGCGTGAGGTGACGGCTGAACTTATGCAGCTGGCCAAAACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAGGAAGGAACCTTGGCTGGTCCGCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGC
CACCATACCTTTCGTATCTTGAGGGCAGTCAAAAACCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGTTTGGTTGAGGTCCTCAATCCGAGTCAAGTTTTCTTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCTATCG
TTGTGACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCTAAG
CGTACGACGACGGGACTTGATTTTAATCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAGCGTGCAGGTCTTCTCTTGCA
AAATCAGGACGCCTATCTCAAATCTGCTGGCGGTGTCAAATTGGATGAACCTGCCATTGACTTGGCAGTTGCAGTTGCCA
TTGCTTCGAGCTATAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTGGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATCGAACAACGTATCAACGAAGCTGCTAAATTGGGCTTTACTAAGATTTATGTACCCAAAAATTCCTT
GACAGGAATCACTCCGCCCAAGGAAATTCAGGTCATTGGCGTAACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(64-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA Streptococcus mitis SK321

99.338

100

0.993
  radA Streptococcus pneumoniae Rx1

99.338

100

0.993
  radA Streptococcus pneumoniae D39

99.338

100

0.993
  radA Streptococcus pneumoniae R6

99.338

100

0.993
  radA Streptococcus pneumoniae TIGR4

99.338

100

0.993
  radA Bacillus subtilis subsp. subtilis str. 168

63.355

100

0.634

Multiple sequence alignment