Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACLV7A_RS00150 Genome accession   NZ_OZ217344
Coordinates   26124..27485 (+) Length   453 a.a.
NCBI ID   WP_411864200.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis E22     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 21124..32485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7A_RS00120 (SMIE22_00190) comW 22543..22779 (+) 237 WP_173213476.1 sigma(X)-activator ComW Regulator
  ACLV7A_RS00125 (SMIE22_00200) - 23010..24296 (+) 1287 WP_001832534.1 adenylosuccinate synthase -
  ACLV7A_RS00130 (SMIE22_00220) tadA 24496..24963 (+) 468 WP_173253439.1 tRNA adenosine(34) deaminase TadA -
  ACLV7A_RS00140 (SMIE22_00240) - 25150..25593 (+) 444 WP_176139895.1 dUTP diphosphatase -
  ACLV7A_RS00145 (SMIE22_00250) - 25544..26110 (+) 567 WP_176139887.1 histidine phosphatase family protein -
  ACLV7A_RS00150 (SMIE22_00260) radA 26124..27485 (+) 1362 WP_411864200.1 DNA repair protein RadA Machinery gene
  ACLV7A_RS00155 (SMIE22_00270) - 27558..28055 (+) 498 WP_004237611.1 beta-class carbonic anhydrase -
  ACLV7A_RS00160 (SMIE22_00280) - 28080..28895 (+) 816 WP_268727419.1 PrsW family intramembrane metalloprotease -
  ACLV7A_RS00165 (SMIE22_00290) - 29041..30009 (+) 969 WP_176139889.1 ribose-phosphate diphosphokinase -
  ACLV7A_RS00170 (SMIE22_00300) - 30127..31122 (+) 996 WP_411863293.1 Rpn family recombination-promoting nuclease/putative transposase -
  ACLV7A_RS00175 - 31121..31420 (+) 300 Protein_27 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49360.84 Da        Isoelectric Point: 6.8555

>NTDB_id=1170249 ACLV7A_RS00150 WP_411864200.1 26124..27485(+) (radA) [Streptococcus mitis isolate S. mitis E22]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVGVTEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTDMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1170249 ACLV7A_RS00150 WP_411864200.1 26124..27485(+) (radA) [Streptococcus mitis isolate S. mitis E22]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGTCCTAACTG
TGGGTCTTGGTCTTCTTTTGTAGAAGAGGTTGGGGTTACCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTGGCTGAGGTGACTTCTATCAACGTTAATCGAACCAAGACGGACATGGAGGAATTCAACCGTGTA
CTTGGAGGTGGAGTGGTACCAGGAAGTCTCGTCCTAATCGGTGGGGATCCTGGAATCGGGAAATCGACTCTTCTCCTACA
AGTCTCAACCCAGCTGTCTCAAGTGGGTACGGTTCTCTACGTCAGCGGGGAGGAGTCTGCCCAGCAGATTAAGCTACGAG
CAGAGCGCTTGGGTGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGCGTTCGAGCTGAGGTGGAG
CGCATCCAACCAGATTTTCTCATCATCGACTCCATCCAGACGATTATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAAGTGCGTGAGGTGACCGCTGAACTGATGCAGCTGGCCAAAACTAATAACATTGCTATCTTTATCGTAGGGCATG
TGACCAAGGAAGGAACCTTGGCTGGTCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCATACCTTCCGTATTCTGAGAGCGGTCAAAAACCGTTTTGGTTCAACAAATGAGATTGGGATTTTTGAGATGCAGTC
GGGTGGTCTGGTTGAGGTCCTCAATCCGAGTCAAGTTTTTCTAGAAGAGCGTTTGGATGGAGCAACTGGCTCGTCAATCG
TTGTGACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTGACACCGACCATGTTTGGAAATGCCAAG
CGTACGACGACAGGACTTGATTTTAATCGTGCTAGTCTGATTATGGCTGTTTTGGAAAAACGTGCAGGTCTTCTCTTGCA
AAATCAGGATGCTTATCTCAAATCTGCTGGCGGTGTCAAATTGGATGAACCTGCGATAGACTTGGCCGTTGCGGTTGCTA
TTGCTTCGAGCTATAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTTGGAGAACTGGGATTGACGGGAGAGATTCGG
CGCGTGAATCGTATCGAGCAGCGCATCAATGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATTACTCCGCCTAAGGAAATTCAGGTTATTGGGGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(64-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

99.117

100

0.991
  radA Streptococcus mitis SK321

99.117

100

0.991
  radA Streptococcus pneumoniae Rx1

99.117

100

0.991
  radA Streptococcus pneumoniae D39

99.117

100

0.991
  radA Streptococcus pneumoniae R6

99.117

100

0.991
  radA Streptococcus pneumoniae TIGR4

99.117

100

0.991
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment