Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACLV65_RS00230 Genome accession   NZ_OZ217342
Coordinates   38354..39715 (+) Length   453 a.a.
NCBI ID   WP_247953655.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis B22     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 33354..44715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV65_RS00195 - 33571..34507 (-) 937 Protein_32 ISL3 family transposase -
  ACLV65_RS00200 (SMIB22_00360) comW 34773..35009 (+) 237 WP_000940045.1 sigma(X)-activator ComW Regulator
  ACLV65_RS00205 (SMIB22_00370) - 35240..36526 (+) 1287 WP_001832534.1 adenylosuccinate synthase -
  ACLV65_RS00210 (SMIB22_00390) tadA 36726..37193 (+) 468 WP_001110112.1 tRNA adenosine(34) deaminase TadA -
  ACLV65_RS00220 (SMIB22_00410) - 37380..37823 (+) 444 WP_084926802.1 dUTP diphosphatase -
  ACLV65_RS00225 (SMIB22_00420) - 37774..38340 (+) 567 WP_411864231.1 histidine phosphatase family protein -
  ACLV65_RS00230 (SMIB22_00430) radA 38354..39715 (+) 1362 WP_247953655.1 DNA repair protein RadA Machinery gene
  ACLV65_RS00235 (SMIB22_00440) - 39788..40285 (+) 498 WP_070528259.1 beta-class carbonic anhydrase -
  ACLV65_RS00240 (SMIB22_00450) - 40310..41125 (+) 816 WP_411864232.1 PrsW family intramembrane metalloprotease -
  ACLV65_RS00245 (SMIB22_00460) - 41271..42239 (+) 969 WP_000010159.1 ribose-phosphate diphosphokinase -
  ACLV65_RS00250 (SMIB22_00470) - 42356..43366 (+) 1011 WP_175880432.1 Rpn family recombination-promoting nuclease/putative transposase -
  ACLV65_RS00255 - 43367..43743 (+) 377 Protein_43 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49415.92 Da        Isoelectric Point: 6.8560

>NTDB_id=1170081 ACLV65_RS00230 WP_247953655.1 38354..39715(+) (radA) [Streptococcus mitis isolate S. mitis B22]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPNFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1170081 ACLV65_RS00230 WP_247953655.1 38354..39715(+) (radA) [Streptococcus mitis isolate S. mitis B22]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGTCCCAACTG
TGGGTCTTGGTCTTCTTTTGTAGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTGGCTGAGGTGACGTCAATCAACGTCAATCGAACCAAGACGGAGATGGAGGAATTTAACCGTGTA
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTACTCATAGGTGGGGATCCTGGGATTGGGAAGTCAACGCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACGGTTCTCTATGTCAGTGGGGAGGAATCTGCCCAGCAGATTAAGCTAAGAG
CAGAGCGTTTGGGAGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGAGCTGAGGTGGAA
AGAATCCAGCCAAACTTCCTCATTATTGACTCCATTCAAACCATTATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTACGTGAGGTGACAGCTGAACTTATGCAACTGGCCAAAACTAATAACATTGCCATCTTTATTGTAGGTCATG
TGACCAAGGAAGGAACATTAGCTGGTCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCATACCTTCCGTATTCTGAGAGCGGTCAAAAACCGTTTTGGTTCAACAAATGAGATTGGGATTTTTGAGATGCAGTC
GGGTGGTCTGGTTGAGGTCCTCAATCCGAGTCAAGTTTTTCTAGAAGAGCGTTTGGATGGAGCAACTGGCTCGTCAATCG
TTGTGACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTGACACCGACCATGTTTGGAAATGCTAAG
CGTACAACGACAGGACTTGATTTTAATCGTGCTAGTCTGATTATGGCTGTTTTGGAAAAACGGGCAGGTCTTCTCTTGCA
AAATCAGGATGCTTATCTTAAATCTGCTGGCGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCCGTTGCGGTTGCTA
TTGCTTCGAGCTATAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTGGGAGAACTGGGCTTGACGGGAGAAATTCGG
CGCGTGAATCGTATCGAGCAGCGCATCAATGAAGCTGCTAAACTGGGTTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCCACCCAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAGGTTTTGAAAAAGGTATTTGCAT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

99.558

100

0.996
  radA Streptococcus mitis SK321

99.558

100

0.996
  radA Streptococcus pneumoniae Rx1

99.558

100

0.996
  radA Streptococcus pneumoniae D39

99.558

100

0.996
  radA Streptococcus pneumoniae R6

99.558

100

0.996
  radA Streptococcus pneumoniae TIGR4

99.558

100

0.996
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment