Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACLV7Q_RS00145 Genome accession   NZ_OZ217341
Coordinates   24562..25923 (+) Length   453 a.a.
NCBI ID   WP_411866013.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis C22     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 19562..30923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7Q_RS00105 - 20185..20481 (-) 297 Protein_14 transposase family protein -
  ACLV7Q_RS00110 - 20611..20839 (-) 229 Protein_15 transposase -
  ACLV7Q_RS00115 (SMIC22_00180) comW 20981..21217 (+) 237 WP_049524667.1 sigma(X)-activator ComW Regulator
  ACLV7Q_RS00120 (SMIC22_00190) - 21448..22734 (+) 1287 WP_001832534.1 adenylosuccinate synthase -
  ACLV7Q_RS00125 (SMIC22_00210) tadA 22934..23401 (+) 468 WP_001110110.1 tRNA adenosine(34) deaminase TadA -
  ACLV7Q_RS00135 (SMIC22_00230) - 23588..24031 (+) 444 WP_000701987.1 dUTP diphosphatase -
  ACLV7Q_RS00140 (SMIC22_00240) - 23982..24548 (+) 567 WP_218757492.1 histidine phosphatase family protein -
  ACLV7Q_RS00145 (SMIC22_00250) radA 24562..25923 (+) 1362 WP_411866013.1 DNA repair protein RadA Machinery gene
  ACLV7Q_RS00150 (SMIC22_00260) - 25996..26493 (+) 498 WP_004237611.1 beta-class carbonic anhydrase -
  ACLV7Q_RS00155 (SMIC22_00270) - 26518..27333 (+) 816 WP_218757493.1 PrsW family intramembrane metalloprotease -
  ACLV7Q_RS00160 (SMIC22_00280) - 27479..28447 (+) 969 WP_000010159.1 ribose-phosphate diphosphokinase -
  ACLV7Q_RS00165 (SMIC22_00290) - 28565..29548 (+) 984 WP_218757494.1 Rpn family recombination-promoting nuclease/putative transposase -
  ACLV7Q_RS00170 (SMIC22_00300) - 29547..29846 (+) 300 Protein_26 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49425.92 Da        Isoelectric Point: 6.6516

>NTDB_id=1169999 ACLV7Q_RS00145 WP_411866013.1 24562..25923(+) (radA) [Streptococcus mitis isolate S. mitis C22]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIHPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1169999 ACLV7Q_RS00145 WP_411866013.1 24562..25923(+) (radA) [Streptococcus mitis isolate S. mitis C22]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGTCCTAACTG
TGGGTCTTGGTCTTCTTTTGTAGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTATCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTGGCTGAGGTGACTTCAATCAACGTCAATCGAACCAAGACAGAGATGGAGGAATTCAACCGTGTA
CTTGGAGGCGGAGTGGTACCGGGAAGTCTCGTTCTTATCGGTGGGGATCCTGGGATTGGGAAATCAACCCTTCTCTTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACGGTTCTCTATGTCAGTGGGGAGGAATCTGCCCAGCAGATTAAACTACGTG
CAGAGCGTTTGGGAGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGAGCTGAAGTGGAG
CGCATCCATCCAGACTTTCTCATTATTGATTCCATCCAGACTATTATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAGGTGACTGCTGAGCTCATGCAGCTGGCCAAAACCAATAATATTGCCATCTTTATCGTAGGTCATG
TGACCAAGGAAGGTACTCTGGCTGGTCCTCGTATGCTAGAGCATATGGTGGATACGGTGCTTTATTTTGAAGGAGAGCGC
CACCATACCTTTCGTATTTTGAGAGCGGTCAAAAACCGTTTTGGCTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
AGGTGGCTTGGTTGAGGTTCTTAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGAGCGACTGGCTCATCAATCG
TTGTGACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTGACACCGACCATGTTTGGAAATGCTAAG
CGTACAACGACAGGACTTGATTTTAATCGTGCTAGTCTGATTATGGCCGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGCGGTGTCAAATTGGATGAACCTGCGATAGACTTGGCCGTTGCGGTTGCTA
TTGCTTCGAGCTACAAGGACAAGCCAACCAATCCTCAGGAATGTTTTGTAGGAGAACTTGGTTTGACAGGAGAGATTAGG
CGCGTGAATCGTATCGAGCAACGCATCAATGAAGCTGCTAAACTGGGTTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCCGCCCAAGGAAATACAGGTCATTGGGGTGACAACGATTCAGGAAGTTTTGAAAAAGGTTTTTGCTT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

99.558

100

0.996
  radA Streptococcus mitis SK321

99.558

100

0.996
  radA Streptococcus pneumoniae Rx1

99.558

100

0.996
  radA Streptococcus pneumoniae D39

99.558

100

0.996
  radA Streptococcus pneumoniae R6

99.558

100

0.996
  radA Streptococcus pneumoniae TIGR4

99.558

100

0.996
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment