Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AB5Y62_RS01255 Genome accession   NZ_OZ078860
Coordinates   278636..279661 (+) Length   341 a.a.
NCBI ID   WP_231901012.1    Uniprot ID   -
Organism   Vibrio splendidus isolate A06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273636..284661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5Y62_RS01235 - 273968..274696 (-) 729 WP_004729672.1 glutathione peroxidase -
  AB5Y62_RS01240 - 274740..274919 (-) 180 WP_017080197.1 hypothetical protein -
  AB5Y62_RS01245 oxyR 274918..275811 (+) 894 WP_076648692.1 DNA-binding transcriptional regulator OxyR -
  AB5Y62_RS01250 - 275923..278484 (-) 2562 WP_019350879.1 penicillin-binding protein 1A -
  AB5Y62_RS01255 pilM 278636..279661 (+) 1026 WP_231901012.1 type IV pilus assembly protein PilM Machinery gene
  AB5Y62_RS01260 pilN 279645..280244 (+) 600 WP_017077615.1 PilN domain-containing protein Machinery gene
  AB5Y62_RS01265 pilO 280237..280866 (+) 630 WP_004729667.1 type 4a pilus biogenesis protein PilO Machinery gene
  AB5Y62_RS01270 pilP 280853..281371 (+) 519 WP_004729666.1 pilus assembly protein PilP Machinery gene
  AB5Y62_RS01275 pilQ 281407..283125 (+) 1719 WP_368084059.1 type IV pilus secretin PilQ Machinery gene
  AB5Y62_RS01280 aroK 283302..283820 (+) 519 WP_004729664.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38112.66 Da        Isoelectric Point: 6.9699

>NTDB_id=1166567 AB5Y62_RS01255 WP_231901012.1 278636..279661(+) (pilM) [Vibrio splendidus isolate A06]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCCNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQMASRLYPEKKNWLLVDVGIDQTSLGIIPQGLAPFYKDIAFGTKDLRSHGAASDIESVFATTEDTH
QFIVNLIEKLKRQLQLYSSGNAQQPISGIWLMGEGASIPMVTEELERQFQLNCESLNPLSLFENKVAKRRRLPTNWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=1166567 AB5Y62_RS01255 WP_231901012.1 278636..279661(+) (pilM) [Vibrio splendidus isolate A06]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGGCTGCCTTTTGGCTGTTGCAACGTCGCTATTTCAGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCGATCTATCAAACCTTTGCTCA
TCAGTCTCCCTTTCCGATAGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTATCAGGTATACGCCACACGTAAAGAAGTGGTGGATAGCCGAGCGGATGCTTTGACCAAAGCTGGTTTTAAACCT
GTCGTGGTAGACACGCAGGCACATGGGTTGCTTAACATCTGGCAAATGGCATCGCGCCTGTACCCCGAAAAGAAAAACTG
GCTGCTGGTGGATGTGGGAATCGATCAAACGTCGTTAGGCATTATTCCACAAGGTTTGGCACCGTTTTATAAAGATATCG
CTTTTGGTACCAAAGATCTTCGTAGCCATGGTGCTGCAAGTGATATCGAGAGTGTGTTCGCCACCACTGAAGATACACAT
CAATTTATTGTTAATTTGATTGAGAAGCTCAAGCGTCAATTACAGCTCTATTCATCAGGAAATGCACAGCAACCTATCTC
AGGGATCTGGCTGATGGGCGAGGGTGCCAGCATTCCTATGGTAACCGAAGAGCTAGAACGTCAATTTCAGTTGAATTGCG
AGTCACTGAACCCTTTGTCTCTATTTGAGAATAAGGTGGCGAAGCGACGTCGATTACCAACGAACTGGCAACATTTCGGT
ATTGCGGCGGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.118

99.707

0.54
  pilM Vibrio campbellii strain DS40M4

47.953

100

0.481

Multiple sequence alignment