Detailed information NTG1165894

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: AB3Y92_RS05545
• Genome accession: NZ_OZ061327
• Coordinates: 1108090..1111788 (+)
• Length: 1232 a.a.
• NCBI ID: WP_342498135.1
• Uniprot ID: -
• Organism: Bacillus pumilus isolate Bacillus pumilus CIRM-BIA2784
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1103090..1116788

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB3Y92_RS05530	-	1103386..1103796 (+)	411	WP_058014363.1	DoxX family protein	-
AB3Y92_RS05535	-	1103992..1104438 (-)	447	WP_061407295.1	ferritin-like domain-containing protein	-
AB3Y92_RS05540	addB	1104603..1108112 (+)	3510	WP_342498134.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
AB3Y92_RS05545	addA	1108090..1111788 (+)	3699	WP_342498135.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
AB3Y92_RS05550	sbcD	1111815..1112990 (+)	1176	WP_058014359.1	exonuclease subunit SbcD	-
AB3Y92_RS05555	-	1112987..1116382 (+)	3396	WP_058014358.1	AAA family ATPase	-
AB3Y92_RS05560	-	1116402..1116701 (+)	300	WP_058014357.1	hypothetical protein	-

Sequence

Protein

• Length: 1232 a.a.
• Molecular weight: 142190.19 Da
• Isoelectric Point: 5.0441

>NTDB_id=1165894 AB3Y92_RS05545 WP_342498135.1 1108090..1111788(+) (addA) [Bacillus pumilus isolate Bacillus pumilus CIRM-BIA2784]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPELPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIISKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQFLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMASVLEEAGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKEQDEIVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESQIGHLLESLQQRDLLTDEQIRSINQEGIASFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYRHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTVDAAESILKKRYETQ
IALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3699 bp

>NTDB_id=1165894 AB3Y92_RS05545 WP_342498135.1 1108090..1111788(+) (addA) [Bacillus pumilus isolate Bacillus pumilus CIRM-BIA2784]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCGGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACTTC
CAGTTGATGTGGACCGTCTGCTTGTTGTAACTTTCACAAATGCTTCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTTCGTTTGGCCG
ATCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAACTGGTTGACCGTTACACGTCTGACCGTCACGACTTAGATTTGCAATGGCTCGTGAAGAGAATTTATGAGTT
TTCAAGATCGCATCCATCCCCTGAGCAATGGATGCGGGCATTTCTTTCACTTTATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGTCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGTGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTTTATTAGAAAAAGCCACAGATGCACGTAATCAAGCGAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTTGAAAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATTGAAACAGAAGCTGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATATCAGGATACAAACCTTGTACAAGAAACCATTTTAAAGCTTGTATCTAAAGGAGA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGGCTGGCAGAGCCTATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACCGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTTCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGTAACTTACAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCGTGGGCTCCGCAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCCGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTCTTGTTCATTTAGATGAAAATGAATTGGCTCTGATTCGAACAAGTGA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCATTTATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAGCTAG
AGCGATTTTTCCAATTCCTGCGCAAGTGGCGTGATTTTTCTATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGTGACACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAACAGCGACAGGCGAATCTTCGTGCCCTTTATGA
CAGGGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGTATGATGACGATCCACAGCAGTAAAGGG
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTATTTACTTGA
TAAAGAACTAGGGTTTGGAAGCAAGTACATCCATCCTGAACTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGAGTCCTATATGTGGCACTCACAAGGGCTAAAGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGTAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGGTATCAATCAAAAACATACTTAGATTTCATTGGACCTGCCCTCATCCGTCATCAAGCGATGGCAT
CTGTTTTAGAAGAAGCAGGAGATGTTGTTCTTTCACATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAGAAGGAGCAAGACGAGATTGTACAAGCATTGATGGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGGCTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCTTATGATGAATCACAGATAGGACACTTGCTCGAATCTTTACAGCAGC
GAGATTTGCTAACAGATGAGCAGATCCGGTCAATTAATCAAGAAGGAATTGCATCTTTCTTCTCTACTTCAATTGGCCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAGGTGTCTTTTAGTATGGTGTTACCTGTTAAAGAGGTATACCGTCATAT
AGATACTGAGGGAGAGCCTGTTCTGATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTACAAGGCAGATATACAGTTGACGCTGCTGAATCGATCTTGAAAAAGCGTTATGAAACCCAA
ATAGCATTATATGCAAAAGCTGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTCGATGGGAA
TTTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1224)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.181	100	0.664