Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AB3Y92_RS05540 Genome accession   NZ_OZ061327
Coordinates   1104603..1108112 (+) Length   1169 a.a.
NCBI ID   WP_342498134.1    Uniprot ID   -
Organism   Bacillus pumilus isolate Bacillus pumilus CIRM-BIA2784     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1099603..1113112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3Y92_RS05510 lepB 1100121..1100633 (+) 513 WP_342498133.1 signal peptidase I -
  AB3Y92_RS05515 - 1100669..1101808 (-) 1140 WP_058014366.1 AbrB family transcriptional regulator -
  AB3Y92_RS05520 - 1102075..1102497 (+) 423 WP_058014365.1 BlaI/MecI/CopY family transcriptional regulator -
  AB3Y92_RS05525 - 1102490..1103329 (+) 840 WP_058014364.1 M56 family metallopeptidase -
  AB3Y92_RS05530 - 1103386..1103796 (+) 411 WP_058014363.1 DoxX family protein -
  AB3Y92_RS05535 - 1103992..1104438 (-) 447 WP_061407295.1 ferritin-like domain-containing protein -
  AB3Y92_RS05540 addB 1104603..1108112 (+) 3510 WP_342498134.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AB3Y92_RS05545 addA 1108090..1111788 (+) 3699 WP_342498135.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AB3Y92_RS05550 sbcD 1111815..1112990 (+) 1176 WP_058014359.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134801.60 Da        Isoelectric Point: 5.2665

>NTDB_id=1165893 AB3Y92_RS05540 WP_342498134.1 1104603..1108112(+) (addB) [Bacillus pumilus isolate Bacillus pumilus CIRM-BIA2784]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISVESMHSEYTEERRAAE
KLHDLHVLYQQMEEHLQDEYVHSEDYLTLLAQQVPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYQLAKECGADISETIFERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFRQTDQEIEMEQMLNDVKEW
IAPPLYQLQKRLKNAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYAASDAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLEDNEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPNRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIYKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVTINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=1165893 AB3Y92_RS05540 WP_342498134.1 1104603..1108112(+) (addB) [Bacillus pumilus isolate Bacillus pumilus CIRM-BIA2784]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCCGGTGGAATGATCAGAGCTAAAGTTTTTAGTTTTACTCGTCTTGCTTGGTCTATTTTACAGCAGACTGGT
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCCAGTGATAAGCCAGGGTTTGTGGAGCAAATCGAAAAAACGATGGCTGAATTTAAAAGATACT
GTATGCTGCCTGAGGATATTGAGAAAATTTCAGTGGAGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAGATGGAGGAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACTCTTCTTGCCCAGCAAGTTCCATCAGCTGAAGAAATAAAAGGAGCACACATTTATATCGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCTTGCTCCATGCTGCAAAAATAACTGCGGCTTTCACAGTAGATCGATCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCTAA
AGAGTGCGGAGCTGACATTTCTGAAACCATTTTTGAAAGAAATCATCGGCACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCAGTACAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGACATCCTTGATTTAGTGAGAGAAAAAGGACTCAGACTCCGAGATATCTCGAT
TGTAGCACGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGACATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAGGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTTGAAATCACTAAGAACAAAGCGAGAGAGCAGGCAGA
TCAGCTCGAAAACTATTGTATTGCTTATGGTGTAAAAGGAGAGCGCTGGACAAATGGTTCCCGGTTTCATTACAGGCGCT
TCCAATCATTAGATGAGGATTTCAGGCAAACAGATCAAGAAATCGAAATGGAACAAATGCTAAATGATGTCAAAGAATGG
ATCGCGCCCCCGCTTTACCAGTTGCAGAAAAGACTTAAAAATGCACAAAAGGTAAGGGACATGGTAGAGGCTGTTTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAACTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCTGTGATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCTTTATTCCAACAGATGATAGATACAGGATTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTACGTTTATCATTGGTGTAAACGATGGCGTTA
TTCCCGCACGCCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGAGAATGGTTAAAACGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAAGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCATCATTTGTATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTTTATTGCCTTCAACACTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTATTAGATCCAGAACAGCTGGAAGACAATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCGTATACTGTCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATTAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTAATCGGGATGTATCAAAAAACATCATGTCAAGTTTGTTCTTTACAAATCGAGCGAAGC
CTTTAAAGCCAAATGTCACAAAAGAGCTTTACGGTGATCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGTTTAAAACTTAAGGATCGACAATTTTACAAATTGGAAGCACCCGATATCGG
CCAGCTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTCACCTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCCTAATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAAGTGAG
TGGATTTTCTCCAGTAGGGCTTGAGTTAGGTTTCGGGGGGCAAGGACCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAACTTGTCGGAAGAATTGACAGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTAACCATTACGTATTCGAAAGAGTGGCTCGGCGTAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCAATTCCGCTTGCAGAAGATGAAATTGAGCAGGAAATATACAAGAAATTTAAAATGAAGGGTTTA
TTGCTTGAAGATGTGGAAGCAGTAAAGCTAATGGATCAAACGCTTGATTCAGGTAGATCGCAAGTCATTCAAGCTGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCGGCAGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GTACATTTGAAGAAGCAGGTGAAAGAATTACAAATGGAGAGGTCACAATTAATCCATATAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCTTTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGAGTGCTCACCTCTGA
AAAGGACGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.064

99.316

0.656

Multiple sequence alignment