Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AU14_RS12070 Genome accession   NZ_CP007151
Coordinates   2607546..2608421 (-) Length   291 a.a.
NCBI ID   WP_041341113.1    Uniprot ID   W5YRL1
Organism   Marinobacter similis strain A3d10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2602546..2613421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AU14_RS12050 (AU14_12465) - 2602714..2604816 (-) 2103 WP_236744036.1 response regulator -
  AU14_RS21695 - 2604813..2604980 (-) 168 WP_236744037.1 hypothetical protein -
  AU14_RS12055 (AU14_12470) tsaA 2605108..2605850 (-) 743 Protein_2652 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  AU14_RS12060 (AU14_12475) - 2605853..2606836 (-) 984 WP_236744038.1 hypothetical protein -
  AU14_RS12065 (AU14_12480) coaE 2606943..2607542 (-) 600 WP_041341111.1 dephospho-CoA kinase -
  AU14_RS12070 (AU14_12485) pilD 2607546..2608421 (-) 876 WP_041341113.1 A24 family peptidase Machinery gene
  AU14_RS12075 (AU14_12490) - 2608488..2609710 (-) 1223 Protein_2656 type II secretion system F family protein -
  AU14_RS12080 (AU14_12495) pilB 2609713..2611424 (-) 1712 Protein_2657 type IV-A pilus assembly ATPase PilB -
  AU14_RS19380 - 2611446..2611748 (-) 303 WP_148312741.1 hypothetical protein -
  AU14_RS12085 (AU14_12500) - 2611837..2612301 (+) 465 WP_041341116.1 pilin -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32244.42 Da        Isoelectric Point: 6.4634

>NTDB_id=116541 AU14_RS12070 WP_041341113.1 2607546..2608421(-) (pilD) [Marinobacter similis strain A3d10]
MLTLDTFLATPWLLFLSVTLLSLCIGSFLNVVILRLPKMMHQDWRCQCEEFLELPETQRKPEERITLSKPASTCPSCGHT
IRAWENIPVISYLVLGGKCASCKTGISPRYPIIEAITALLSIATVAILGPTEAALWSLLLVWSLVALTVIDFDTQLLPDS
ITLPLLWLGLVLNYFDLLTDFTSAFWGAVWGYLSLWSVYWLFKLVTGKEGMGHGDFKLLAALGAWLGWQLLPAVILLSSV
VGAVVGISLMILKKHGREVPIPFGPYLAAAGLLCLWFGTEIQRFWFGFLGV

Nucleotide


Download         Length: 876 bp        

>NTDB_id=116541 AU14_RS12070 WP_041341113.1 2607546..2608421(-) (pilD) [Marinobacter similis strain A3d10]
ATGCTCACACTAGACACATTCCTGGCGACACCCTGGCTACTGTTCCTGTCCGTCACCCTGCTATCCCTCTGCATCGGCAG
CTTTCTCAACGTGGTCATCCTGCGCCTGCCGAAGATGATGCATCAGGACTGGCGCTGCCAGTGCGAAGAATTCCTGGAGC
TGCCTGAAACCCAGAGAAAGCCGGAAGAGCGGATCACCCTGTCCAAACCGGCATCGACCTGCCCTTCCTGTGGCCACACC
ATTCGGGCCTGGGAGAACATTCCGGTGATCAGCTACCTGGTTCTTGGCGGCAAGTGCGCCTCCTGCAAGACCGGCATTTC
CCCCCGCTATCCGATCATTGAAGCGATCACTGCCCTGCTCTCCATTGCCACCGTCGCAATCCTTGGCCCAACCGAGGCCG
CGCTTTGGTCGTTGTTGCTGGTGTGGTCCCTGGTGGCTTTGACGGTGATCGACTTCGACACCCAACTGCTACCGGACAGC
ATTACCCTGCCCCTCCTCTGGCTCGGCCTGGTGCTGAATTACTTTGACTTGCTGACCGACTTCACCAGCGCGTTCTGGGG
CGCAGTCTGGGGCTACTTGTCCCTATGGTCGGTGTACTGGCTGTTCAAACTGGTCACTGGCAAGGAAGGTATGGGCCATG
GTGACTTCAAACTACTGGCCGCACTAGGCGCGTGGCTGGGCTGGCAGCTGCTGCCAGCAGTGATTCTGCTGTCTTCGGTG
GTTGGCGCAGTGGTTGGCATCAGCCTGATGATTCTCAAAAAGCATGGCCGCGAGGTGCCAATCCCGTTTGGCCCCTACCT
TGCGGCTGCGGGCCTGCTGTGCCTCTGGTTTGGAACCGAGATCCAACGCTTCTGGTTTGGCTTTTTAGGTGTGTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-125)

Type IV leader peptidase family (Peptidase_A24)

(138-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W5YRL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

55.109

94.158

0.519
  pilD Acinetobacter nosocomialis M2

54.38

94.158

0.512
  pilD Vibrio cholerae strain A1552

51.916

98.625

0.512
  pilD Vibrio campbellii strain DS40M4

50.175

97.938

0.491
  pilD Neisseria gonorrhoeae MS11

49.802

86.942

0.433

Multiple sequence alignment