Detailed information NTG0116511

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AI22_RS09465
• Genome accession: NZ_CP007147
• Coordinates: 2061860..2062732 (-)
• Length: 290 a.a.
• NCBI ID: WP_025324685.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa YL84
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2059125..2067810	2061860..2062732	within	0

Gene organization within MGE regions

Location: 2059125..2067810

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AI22_RS09435 (AI22_09470)	-	2059125..2059550 (+)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
AI22_RS09440 (AI22_09475)	-	2059547..2060017 (+)	471	WP_003103866.1	hypothetical protein	-
AI22_RS09445 (AI22_09480)	-	2060014..2060703 (+)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
AI22_RS09450 (AI22_09485)	-	2060809..2061018 (+)	210	WP_003094660.1	hypothetical protein	-
AI22_RS09455 (AI22_09490)	yacG	2061055..2061255 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
AI22_RS09460 (AI22_09495)	coaE	2061252..2061863 (-)	612	WP_003112838.1	dephospho-CoA kinase	-
AI22_RS09465 (AI22_09500)	pilD	2061860..2062732 (-)	873	WP_025324685.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
AI22_RS09470 (AI22_09505)	pilC	2062733..2063950 (-)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
AI22_RS09475 (AI22_09510)	pilB	2063954..2065654 (-)	1701	WP_003107297.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AI22_RS09480 (AI22_09515)	-	2065885..2066349 (+)	465	WP_003122079.1	pilin	-
AI22_RS09485 (AI22_09520)	-	2066425..2067810 (+)	1386	WP_025324686.1	O-antigen ligase family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31875.94 Da
• Isoelectric Point: 7.7068

>NTDB_id=116511 AI22_RS09465 WP_025324685.1 2061860..2062732(-) (pilD) [Pseudomonas aeruginosa YL84]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDMLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=116511 AI22_RS09465 WP_025324685.1 2061860..2062732(-) (pilD) [Pseudomonas aeruginosa YL84]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCTATCCTCCTTGGCCTGCTGGTTGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACATGCTGGTG
CTACCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCTTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.714	96.552	0.538
pilD	Vibrio campbellii strain DS40M4	53.004	97.586	0.517
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	49.281	95.862	0.472