Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   U9966_RS09870 Genome accession   NZ_OY856306
Coordinates   2156712..2157281 (+) Length   189 a.a.
NCBI ID   WP_211599132.1    Uniprot ID   -
Organism   Pasteurella atlantica isolate PAF6K1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2151712..2162281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9966_RS09850 - 2152456..2153781 (-) 1326 WP_306346402.1 patatin-like phospholipase family protein -
  U9966_RS09855 yfaE 2153881..2154129 (-) 249 WP_211599103.1 class I ribonucleotide reductase maintenance protein YfaE -
  U9966_RS09860 nrdB 2154129..2155259 (-) 1131 WP_306346403.1 class Ia ribonucleoside-diphosphate reductase subunit beta -
  U9966_RS09865 - 2155595..2156698 (+) 1104 WP_306346480.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  U9966_RS09870 pilF 2156712..2157281 (+) 570 WP_211599132.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  U9966_RS09875 sohB 2157373..2158455 (-) 1083 WP_306346404.1 protease SohB -
  U9966_RS09880 folC 2158474..2159775 (-) 1302 WP_306346405.1 bifunctional tetrahydrofolate synthase/dihydrofolate synthase -
  U9966_RS09885 accD 2159775..2160683 (-) 909 WP_306346406.1 acetyl-CoA carboxylase, carboxyltransferase subunit beta -

Sequence

Protein


Download         Length: 189 a.a.        Molecular weight: 21611.58 Da        Isoelectric Point: 9.1873

>NTDB_id=1162157 U9966_RS09870 WP_211599132.1 2156712..2157281(+) (pilF) [Pasteurella atlantica isolate PAF6K1]
MKIANFYSNLTACIIALTLTGCVSTSTEHSINKTEAMQARLNLALAYLKENNFPKAKLNIDKALEHNNQHYLPYSVLAYY
YQQTGKVSDASQTYQQAVTLSQKFTKDHQPRPDILNNYGTFLCKQAQFKQAYNLFEQALTSKQSYYHQADTLENIILCAK
GEKNHTQMNNALIQLRKIDKERAEKISSM

Nucleotide


Download         Length: 570 bp        

>NTDB_id=1162157 U9966_RS09870 WP_211599132.1 2156712..2157281(+) (pilF) [Pasteurella atlantica isolate PAF6K1]
ATGAAAATTGCAAATTTTTATTCAAATCTTACCGCTTGTATAATTGCTTTGACTTTGACAGGTTGTGTTTCTACATCAAC
AGAACACTCAATAAACAAAACAGAAGCAATGCAAGCAAGACTCAACCTTGCACTTGCTTATTTAAAAGAAAATAATTTTC
CAAAAGCAAAATTAAATATCGACAAAGCACTGGAACATAACAATCAGCACTATTTACCTTATTCAGTACTTGCTTATTAC
TATCAACAGACAGGTAAAGTTTCAGATGCATCACAAACCTATCAACAAGCGGTTACATTAAGTCAAAAATTTACAAAAGA
TCACCAACCTCGTCCTGATATATTAAATAATTATGGGACTTTTTTATGTAAACAAGCCCAATTTAAACAAGCTTATAATT
TATTTGAACAAGCATTAACAAGTAAACAATCTTATTATCATCAAGCTGATACGCTAGAAAATATTATACTTTGTGCTAAA
GGTGAGAAAAACCATACTCAAATGAATAACGCATTAATTCAATTAAGAAAAATAGATAAAGAACGAGCAGAGAAAATTAG
TTCAATGTGA

Domains

Predicted by InterproScan.

Coatomer epsilon subunit (Coatomer_E)

(31-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

58.602

98.413

0.577
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.111

95.238

0.439

Multiple sequence alignment