Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP47_RS02035 Genome accession   NZ_OX419577
Coordinates   383399..383848 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis isolate NRS6120     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 378399..388848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP47_RS01995 - 378720..378917 (+) 198 WP_121509395.1 hypothetical protein -
  KJP47_RS02000 yckC 378937..379391 (+) 455 Protein_351 RDD family protein -
  KJP47_RS02005 (NRS6120_02125) yckD 379473..379805 (+) 333 WP_213382693.1 YckD family protein -
  KJP47_RS02010 (NRS6120_02130) - 379933..380355 (+) 423 WP_213382735.1 MarR family transcriptional regulator -
  KJP47_RS02015 (NRS6120_02135) - 380343..380684 (+) 342 WP_032724911.1 DUF3147 family protein -
  KJP47_RS02020 (NRS6120_02140) - 380703..381431 (+) 729 WP_021481826.1 AAA family ATPase -
  KJP47_RS02025 (NRS6120_02145) bglC 381504..382937 (+) 1434 WP_213382691.1 family 1 glycosylhydrolase -
  KJP47_RS02030 (NRS6120_02150) nin/comJ 382974..383372 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  KJP47_RS02035 (NRS6120_02155) nucA/comI 383399..383848 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  KJP47_RS02040 (NRS6120_02160) tlpC 384016..385737 (-) 1722 WP_213382690.1 methyl-accepting chemotaxis protein -
  KJP47_RS02045 (NRS6120_02165) hxlB 385847..386404 (-) 558 WP_017696129.1 6-phospho-3-hexuloisomerase -
  KJP47_RS02050 (NRS6120_02170) hxlA 386410..387042 (-) 633 WP_017696130.1 3-hexulose-6-phosphate synthase -
  KJP47_RS02055 (NRS6120_02175) hxlR 387274..387636 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=1157718 KJP47_RS02035 WP_014475770.1 383399..383848(-) (nucA/comI) [Bacillus subtilis isolate NRS6120]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1157718 KJP47_RS02035 WP_014475770.1 383399..383848(-) (nucA/comI) [Bacillus subtilis isolate NRS6120]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGATGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987

Multiple sequence alignment