Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   KJP57_RS13750 Genome accession   NZ_OX419573
Coordinates   2609991..2610374 (-) Length   127 a.a.
NCBI ID   WP_128738019.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6085     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2604991..2615374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP57_RS13710 (NRS6085_13720) sinI 2605924..2606097 (+) 174 WP_003230187.1 anti-repressor SinI family protein Regulator
  KJP57_RS13715 (NRS6085_13725) sinR 2606131..2606466 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  KJP57_RS13720 (NRS6085_13730) tasA 2606559..2607344 (-) 786 WP_128738018.1 biofilm matrix protein TasA -
  KJP57_RS13725 (NRS6085_13735) sipW 2607408..2607980 (-) 573 WP_128738626.1 signal peptidase I -
  KJP57_RS13730 (NRS6085_13740) tapA 2607964..2608725 (-) 762 WP_101169542.1 amyloid fiber anchoring/assembly protein TapA -
  KJP57_RS13735 (NRS6085_13745) yqzG 2608998..2609324 (+) 327 WP_024573388.1 YqzG/YhdC family protein -
  KJP57_RS13740 (NRS6085_13750) spoIIT 2609366..2609545 (-) 180 WP_014480252.1 YqzE family protein -
  KJP57_RS13745 (NRS6085_13755) comGG 2609616..2609990 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  KJP57_RS13750 (NRS6085_13760) comGF 2609991..2610374 (-) 384 WP_128738019.1 ComG operon protein ComGF Machinery gene
  KJP57_RS13755 (NRS6085_13765) comGE 2610400..2610747 (-) 348 WP_128738020.1 ComG operon protein 5 Machinery gene
  KJP57_RS13760 (NRS6085_13770) comGD 2610731..2611162 (-) 432 WP_024573390.1 comG operon protein ComGD Machinery gene
  KJP57_RS13765 (NRS6085_13775) comGC 2611152..2611448 (-) 297 WP_024573391.1 comG operon protein ComGC Machinery gene
  KJP57_RS13770 (NRS6085_13780) comGB 2611462..2612499 (-) 1038 WP_128738021.1 comG operon protein ComGB Machinery gene
  KJP57_RS13775 (NRS6085_13785) comGA 2612486..2613556 (-) 1071 WP_128738022.1 competence protein ComGA Machinery gene
  KJP57_RS13780 - 2613767..2613892 (-) 126 WP_003230155.1 hypothetical protein -
  KJP57_RS13785 (NRS6085_13790) corA 2613958..2614911 (-) 954 WP_213407806.1 magnesium transporter CorA family protein -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14354.51 Da        Isoelectric Point: 6.3242

>NTDB_id=1157504 KJP57_RS13750 WP_128738019.1 2609991..2610374(-) (comGF) [Bacillus subtilis isolate NRS6085]
MLISGSLAAIFHLFLSRQQEYDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFEIYHSMIRKRVDG
KGHVPILDHITAMKADIKNGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=1157504 KJP57_RS13750 WP_128738019.1 2609991..2610374(-) (comGF) [Bacillus subtilis isolate NRS6085]
TTGCTCATATCAGGATCGTTAGCTGCGATTTTCCATCTGTTTTTGTCTCGACAGCAGGAATATGACGGTTTCACACAGCA
AGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAGTCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGGCAGGACATCCGTTTCGAAATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATATTAAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAGGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

96.85

100

0.969

Multiple sequence alignment