Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP64_RS01965 Genome accession   NZ_OX419565
Coordinates   365774..366223 (-) Length   149 a.a.
NCBI ID   WP_033883649.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6145     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 360774..371223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP64_RS01930 (NRS6145_01920) yckA 361043..361723 (-) 681 WP_213380016.1 amino acid ABC transporter permease -
  KJP64_RS01935 (NRS6145_01925) yckB 361733..362596 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  KJP64_RS01940 - 362758..362955 (+) 198 Protein_341 hypothetical protein -
  KJP64_RS01945 (NRS6145_01935) - 362992..363312 (+) 321 Protein_342 RDD family protein -
  KJP64_RS01950 (NRS6145_01940) yckD 363394..363726 (+) 333 WP_213380012.1 YckD family protein -
  KJP64_RS01955 (NRS6145_01945) bglC 363879..365312 (+) 1434 WP_213380010.1 family 1 glycosylhydrolase -
  KJP64_RS01960 (NRS6145_01950) nin/comJ 365349..365747 (-) 399 WP_015252845.1 DNA-entry nuclease inhibitor Regulator
  KJP64_RS01965 (NRS6145_01955) nucA/comI 365774..366223 (-) 450 WP_033883649.1 DNA-entry nuclease Machinery gene
  KJP64_RS01970 (NRS6145_01960) tlpC 366391..368112 (-) 1722 WP_213380008.1 methyl-accepting chemotaxis protein -
  KJP64_RS01975 (NRS6145_01965) hxlB 368223..368780 (-) 558 WP_024572182.1 6-phospho-3-hexuloisomerase -
  KJP64_RS01980 (NRS6145_01970) hxlA 368786..369418 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  KJP64_RS01985 (NRS6145_01975) hxlR 369651..370013 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16454.42 Da        Isoelectric Point: 4.5282

>NTDB_id=1157126 KJP64_RS01965 WP_033883649.1 365774..366223(-) (nucA/comI) [Bacillus subtilis isolate NRS6145]
MNITTDIIKTILLVIVIIAAAAIGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1157126 KJP64_RS01965 WP_033883649.1 365774..366223(-) (nucA/comI) [Bacillus subtilis isolate NRS6145]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTATCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment