Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KJP48_RS13390 Genome accession   NZ_OX419564
Coordinates   2550329..2551399 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis isolate NRS6127     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2545329..2556399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP48_RS13345 (NRS6127_13260) tapA 2545807..2546568 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  KJP48_RS13350 (NRS6127_13265) yqzG 2546840..2547166 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  KJP48_RS13355 (NRS6127_13270) spoIIT 2547208..2547387 (-) 180 WP_029726723.1 YqzE family protein -
  KJP48_RS13360 (NRS6127_13275) comGG 2547459..2547833 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  KJP48_RS13365 comGF 2547834..2548217 (-) 384 WP_029726721.1 ComG operon protein ComGF Machinery gene
  KJP48_RS13370 (NRS6127_13285) comGE 2548243..2548590 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  KJP48_RS13375 (NRS6127_13290) comGD 2548574..2549005 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  KJP48_RS13380 (NRS6127_13295) comGC 2548995..2549291 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  KJP48_RS13385 (NRS6127_13300) comGB 2549305..2550342 (-) 1038 WP_029726718.1 comG operon protein ComGB Machinery gene
  KJP48_RS13390 (NRS6127_13305) comGA 2550329..2551399 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  KJP48_RS13395 (NRS6127_13315) - 2551612..2551809 (-) 198 WP_029726717.1 hypothetical protein -
  KJP48_RS13400 (NRS6127_13320) corA 2551811..2552764 (-) 954 WP_004399136.1 magnesium transporter CorA -
  KJP48_RS13405 (NRS6127_13325) yqhB 2552907..2554235 (+) 1329 WP_029726716.1 hemolysin family protein -
  KJP48_RS13410 (NRS6127_13330) rsbRD 2554288..2555124 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -
  KJP48_RS13420 (NRS6127_13340) mgsR 2555348..2555728 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  KJP48_RS13425 (NRS6127_13345) yqgY 2555960..2556205 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=1157088 KJP48_RS13390 WP_004399124.1 2550329..2551399(-) (comGA) [Bacillus subtilis isolate NRS6127]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1157088 KJP48_RS13390 WP_004399124.1 2550329..2551399(-) (comGA) [Bacillus subtilis isolate NRS6127]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment