Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   BAMY6614_RS14990 Genome accession   NZ_CP006960
Coordinates   3169090..3170127 (-) Length   345 a.a.
NCBI ID   WP_061862392.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens UMAF6614     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3164090..3175127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAMY6614_RS14940 (BAMY6614_15785) tasA 3164323..3165108 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  BAMY6614_RS14945 (BAMY6614_15790) sipW 3165173..3165757 (-) 585 WP_014418370.1 signal peptidase I SipW -
  BAMY6614_RS14950 (BAMY6614_15795) tapA 3165729..3166400 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  BAMY6614_RS14955 (BAMY6614_15800) - 3166659..3166988 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  BAMY6614_RS14960 (BAMY6614_15805) - 3167029..3167208 (-) 180 WP_003153093.1 YqzE family protein -
  BAMY6614_RS14965 (BAMY6614_15810) comGG 3167265..3167642 (-) 378 WP_061862389.1 competence type IV pilus minor pilin ComGG Machinery gene
  BAMY6614_RS14970 (BAMY6614_15815) comGF 3167643..3168038 (-) 396 WP_061862390.1 competence type IV pilus minor pilin ComGF -
  BAMY6614_RS14975 (BAMY6614_15820) comGE 3168052..3168366 (-) 315 WP_061862391.1 competence type IV pilus minor pilin ComGE Machinery gene
  BAMY6614_RS14980 (BAMY6614_15825) comGD 3168350..3168787 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  BAMY6614_RS14985 (BAMY6614_15830) comGC 3168777..3169043 (-) 267 WP_050496152.1 competence type IV pilus major pilin ComGC Machinery gene
  BAMY6614_RS14990 (BAMY6614_15835) comGB 3169090..3170127 (-) 1038 WP_061862392.1 competence type IV pilus assembly protein ComGB Machinery gene
  BAMY6614_RS14995 (BAMY6614_15840) comGA 3170114..3171184 (-) 1071 WP_061862635.1 competence type IV pilus ATPase ComGA Machinery gene
  BAMY6614_RS15000 (BAMY6614_15845) - 3171377..3172327 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  BAMY6614_RS15005 (BAMY6614_15850) - 3172473..3173774 (+) 1302 WP_061862393.1 hemolysin family protein -
  BAMY6614_RS15010 (BAMY6614_15855) - 3173812..3174642 (-) 831 WP_021494316.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39611.92 Da        Isoelectric Point: 9.7504

>NTDB_id=115342 BAMY6614_RS14990 WP_061862392.1 3169090..3170127(-) (comGB) [Bacillus amyloliquefaciens UMAF6614]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIIVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGRLHRELYTYSQFMKERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=115342 BAMY6614_RS14990 WP_061862392.1 3169090..3170127(-) (comGB) [Bacillus amyloliquefaciens UMAF6614]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GACGGCTGAGGGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCACAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACCTCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTGATTATTGTGAAAATCCCCCTATTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGCTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCGGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGAAGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

66.254

93.623

0.62

Multiple sequence alignment