Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   F544_RS08965 Genome accession   NZ_CP006956
Coordinates   1964141..1964713 (-) Length   190 a.a.
NCBI ID   WP_025289759.1    Uniprot ID   W0RCB9
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-190     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1959141..1969713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F544_RS08935 (F544_18040) - 1959453..1959617 (-) 165 WP_015433237.1 Trm112 family protein -
  F544_RS08940 (F544_18050) lpxK 1959610..1960599 (-) 990 WP_025289757.1 tetraacyldisaccharide 4'-kinase -
  F544_RS08945 (F544_18060) clpX 1960769..1962022 (-) 1254 WP_025289758.1 ATP-dependent protease ATP-binding subunit ClpX -
  F544_RS08950 (F544_18070) clpP 1962022..1962612 (-) 591 WP_025266793.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  F544_RS08955 (F544_18080) - 1962720..1963118 (+) 399 WP_015433242.1 SufE family protein -
  F544_RS08960 (F544_18090) - 1963100..1964086 (-) 987 WP_015433243.1 AEC family transporter -
  F544_RS08965 (F544_18100) pilF 1964141..1964713 (-) 573 WP_025289759.1 tetratricopeptide repeat protein Machinery gene
  F544_RS08970 (F544_18110) - 1964812..1965375 (-) 564 WP_025289760.1 anthranilate synthase component II -
  F544_RS08975 (F544_18120) rmuC 1965380..1967008 (-) 1629 WP_025289761.1 DNA recombination protein RmuC -
  F544_RS08980 (F544_18130) ung 1967077..1967739 (-) 663 WP_025289762.1 uracil-DNA glycosylase -
  F544_RS08985 (F544_18140) grcA 1967856..1968239 (+) 384 WP_015433248.1 autonomous glycyl radical cofactor GrcA -
  F544_RS08990 (F544_18150) rpsO 1968367..1968636 (-) 270 WP_015433249.1 30S ribosomal protein S15 -

Sequence

Protein


Download         Length: 190 a.a.        Molecular weight: 21443.54 Da        Isoelectric Point: 7.3201

>NTDB_id=115255 F544_RS08965 WP_025289759.1 1964141..1964713(-) (pilF) [Bibersteinia trehalosi USDA-ARS-USMARC-190]
MFFAKLPQYLSACLLIFLFSGCVNLQTSADFDRDEALIARASLALAYLEQQDFSKAKENIEKALSHNSQHYLAHLVAAYY
YQQLSDVQHAEQSYQAAIRLSHQIKQNSPLPEVLNNYGTFLCQQGKFVQAYTQFEQALTGEKAYLRQGDTLENMVLCAKQ
ANQLEKMAQALARLALLDAERATKLAKIVK

Nucleotide


Download         Length: 573 bp        

>NTDB_id=115255 F544_RS08965 WP_025289759.1 1964141..1964713(-) (pilF) [Bibersteinia trehalosi USDA-ARS-USMARC-190]
ATGTTTTTTGCAAAATTGCCTCAATATTTAAGCGCTTGCTTGCTGATTTTTTTATTCAGCGGTTGCGTAAACCTACAAAC
GTCAGCTGATTTTGATAGAGATGAAGCACTAATAGCGCGAGCAAGCCTTGCTCTTGCTTATCTTGAACAGCAGGATTTCT
CAAAAGCGAAAGAGAATATAGAGAAAGCCTTGAGCCACAATAGCCAACATTATTTAGCGCACCTTGTCGCCGCTTATTAC
TATCAGCAACTTAGCGATGTTCAGCATGCAGAGCAAAGTTATCAGGCAGCGATTCGTTTAAGCCATCAGATTAAGCAAAA
TTCACCATTGCCTGAGGTGCTCAATAACTATGGGACATTTTTATGCCAACAAGGTAAATTTGTTCAAGCTTATACGCAAT
TTGAGCAAGCACTTACAGGAGAAAAAGCTTATCTACGCCAAGGCGATACATTAGAAAATATGGTATTGTGTGCCAAGCAG
GCTAACCAGTTAGAGAAAATGGCTCAGGCACTAGCGCGCTTAGCGCTATTAGATGCAGAACGGGCTACAAAACTTGCAAA
AATTGTCAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat-like domain (TPR_21)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0RCB9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

53.763

97.895

0.526
  pilF/pilF2 Haemophilus influenzae Rd KW20

43.182

92.632

0.4

Multiple sequence alignment