Detailed information NTG1152287

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OC617_RS02285
• Genome accession: NZ_OW443147
• Coordinates: 475099..475974 (-)
• Length: 291 a.a.
• NCBI ID: WP_000418747.1
• Uniprot ID: H9L4S3
• Organism: Vibrio cholerae strain CNRVC190243 isolate YE-NCPHL-19014-PI
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 470099..480974

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OC617_RS02265 (CNRVC190243H_00451)	parC	471049..473334 (+)	2286	WP_000102502.1	DNA topoisomerase IV subunit A	-
OC617_RS02270 (CNRVC190243H_00452)	yacG	473383..473580 (-)	198	WP_000162868.1	DNA gyrase inhibitor YacG	-
OC617_RS02275 (CNRVC190243H_00453)	zapD	473722..474462 (-)	741	WP_080876404.1	cell division protein ZapD	-
OC617_RS02280 (CNRVC190243H_00454)	coaE	474494..475102 (-)	609	WP_000011557.1	dephospho-CoA kinase	-
OC617_RS02285 (CNRVC190243H_00455)	pilD	475099..475974 (-)	876	WP_000418747.1	A24 family peptidase	Machinery gene
OC617_RS02290 (CNRVC190243H_00456)	pilC	476032..477258 (-)	1227	WP_000648511.1	type II secretion system F family protein	Machinery gene
OC617_RS02295 (CNRVC190243H_00457)	pilB	477301..478989 (-)	1689	WP_000957200.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OC617_RS02300 (CNRVC190243H_00458)	pilA	478989..479450 (-)	462	WP_000649326.1	pilin	Machinery gene
OC617_RS02305 (CNRVC190243H_00459)	nadC	479695..480585 (-)	891	WP_000665274.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32433.69 Da
• Isoelectric Point: 7.1618

>NTDB_id=1152287 OC617_RS02285 WP_000418747.1 475099..475974(-) (pilD) [Vibrio cholerae strain CNRVC190243 isolate YE-NCPHL-19014-PI]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide

• Length: 876 bp

>NTDB_id=1152287 OC617_RS02285 WP_000418747.1 475099..475974(-) (pilD) [Vibrio cholerae strain CNRVC190243 isolate YE-NCPHL-19014-PI]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGTCAAACGCCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTAGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCCACTCACTTTCCGTTCGGAGTTTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTGTTGATTGCGGCAACCTTCATCGACTTCGATACCCTGTTGTTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCTGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCGATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCTCTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: H9L4S3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	100	100	1
pilD	Vibrio campbellii strain DS40M4	75.347	98.969	0.746
pilD	Acinetobacter nosocomialis M2	53.676	93.471	0.502
pilD	Acinetobacter baumannii D1279779	53.309	93.471	0.498
pilD	Neisseria gonorrhoeae MS11	44.792	98.969	0.443