Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QM552_RS04420 Genome accession   NZ_OW011623
Coordinates   944124..945584 (+) Length   486 a.a.
NCBI ID   WP_004259803.1    Uniprot ID   N6Y4X4
Organism   Thauera humireducens strain Piv1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 939124..950584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QM552_RS04390 (C4PIVTH_0909) thiL 939755..940714 (+) 960 WP_282809579.1 thiamine-phosphate kinase -
  QM552_RS04395 (C4PIVTH_0910) - 940790..941272 (+) 483 WP_004259814.1 phosphatidylglycerophosphatase A -
  QM552_RS04400 (C4PIVTH_0911) - 941292..941777 (+) 486 WP_004259811.1 CinA family protein -
  QM552_RS04405 (C4PIVTH_0912) recA 941911..942945 (+) 1035 WP_004259809.1 recombinase RecA Machinery gene
  QM552_RS04410 (C4PIVTH_0913) recX 942958..943407 (+) 450 WP_004259807.1 recombination regulator RecX -
  QM552_RS04415 (C4PIVTH_0914) lptB 943394..944116 (+) 723 WP_004259805.1 LPS export ABC transporter ATP-binding protein -
  QM552_RS04420 (C4PIVTH_0915) rpoN/rpoN1 944124..945584 (+) 1461 WP_004259803.1 RNA polymerase factor sigma-54 Machinery gene
  QM552_RS04425 (C4PIVTH_0916) hpf 945602..945925 (+) 324 WP_004259801.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  QM552_RS04430 (C4PIVTH_0917) ptsN 946062..946547 (+) 486 WP_004259799.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QM552_RS04435 (C4PIVTH_0918) hprK 946516..947463 (+) 948 WP_004259797.1 HPr(Ser) kinase/phosphatase -
  QM552_RS04440 (C4PIVTH_0919) ilvA 947580..949100 (+) 1521 WP_004259793.1 threonine ammonia-lyase, biosynthetic -
  QM552_RS04445 (C4PIVTH_0920) - 949113..949745 (-) 633 WP_282809580.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 486 a.a.        Molecular weight: 54770.72 Da        Isoelectric Point: 4.8867

>NTDB_id=1152191 QM552_RS04420 WP_004259803.1 944124..945584(+) (rpoN/rpoN1) [Thauera humireducens strain Piv1]
MKPSLQLKLSQHLTLTPQLQQSIKLLQLSTLELNQEIERFLLENPMLEREEIDDFVPQPGLATSTPPAERERERERETSA
EATSDGEAGKANDREDNEEGIDWSNVGSGAQAKRDDDDDTDFQEFQAAELSLRDHLDEQVALSPLSDRDRALVRFIIEAL
DDDGYLHQPLEELLELLPPELDIDLDELSIALRHVQHLDPAGIGARSPQECLSLQLRAMPETPTQMLALEIVDQHLELLA
ERNFARLKKHTGCDDDQLRAAHALICSLDPHPGSRHSVSETRYVLPDVIVRKIRGRWTVSLNQDAMPRLRVNRLYASLLQ
QNRGQGGGLTGQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGDVAMRPLTLREIADQLELHESTVSRVTTQKYM
ATPRGVFELKYFFGSHVSTDTGGAASSTAIRALIRQLVDAEDSKKPLSDARIAEVLGQQGIVVARRTIAKYRESLNIPPV
SLRKSI

Nucleotide


Download         Length: 1461 bp        

>NTDB_id=1152191 QM552_RS04420 WP_004259803.1 944124..945584(+) (rpoN/rpoN1) [Thauera humireducens strain Piv1]
ATGAAACCCAGCCTCCAGCTCAAGCTCTCGCAGCACCTCACGCTGACGCCTCAGCTGCAGCAGTCGATCAAGCTGCTGCA
GCTGTCCACGCTCGAGCTGAACCAGGAGATCGAGCGCTTCCTGCTCGAGAACCCGATGCTCGAGCGCGAGGAGATCGACG
ACTTCGTGCCGCAGCCCGGTCTCGCCACCAGCACCCCGCCTGCCGAACGTGAGCGCGAGCGCGAACGCGAAACCAGCGCC
GAGGCCACCAGCGACGGCGAAGCAGGAAAGGCGAACGATCGCGAGGACAACGAGGAAGGCATCGACTGGTCCAACGTCGG
CAGTGGCGCCCAGGCCAAGCGCGACGATGACGACGACACCGACTTCCAGGAGTTCCAGGCGGCCGAGCTGTCGCTTCGCG
ACCACCTCGACGAGCAGGTCGCGCTGTCGCCGCTCTCCGACCGCGACCGCGCGCTGGTGCGCTTCATCATCGAGGCGCTG
GACGACGACGGCTACCTGCACCAGCCGCTCGAGGAGTTGCTCGAGTTGCTGCCACCCGAGCTCGACATCGACCTCGACGA
GCTTTCCATCGCGCTGCGCCACGTACAGCACCTCGACCCTGCCGGCATCGGCGCACGCAGCCCGCAGGAATGCCTGTCGC
TGCAGCTGCGAGCGATGCCCGAGACGCCGACGCAGATGCTCGCGCTCGAGATCGTTGACCAGCATCTCGAACTGCTCGCC
GAGCGCAACTTCGCGCGCCTCAAGAAACACACCGGTTGCGACGACGACCAGCTGCGCGCTGCCCACGCTCTGATCTGCAG
CCTCGACCCGCATCCGGGCTCGCGCCACTCCGTCTCCGAGACGCGCTACGTGCTGCCTGACGTCATCGTGCGCAAGATCC
GCGGACGCTGGACCGTCAGCCTCAACCAGGACGCGATGCCGCGCCTGCGCGTCAACCGGCTCTACGCCAGCCTGCTGCAG
CAGAACCGCGGCCAGGGCGGCGGCCTGACCGGCCAGTTACAGGAAGCACGCTGGCTGATCAAGAACGTCCAGCAGCGTTT
CGACACCATCCTGCGGGTCTCCCAAGCCATCGTTGACCAGCAGCGGCAGTTCTTCGATCATGGAGACGTGGCGATGCGGC
CACTCACTCTCCGGGAGATCGCGGACCAGCTCGAACTGCACGAATCCACCGTTTCGCGCGTCACGACGCAGAAATACATG
GCGACGCCGCGCGGTGTGTTCGAACTCAAGTATTTCTTCGGCAGCCACGTCTCCACCGACACTGGCGGAGCGGCCTCTTC
CACGGCGATTCGGGCGCTGATTCGTCAACTGGTGGACGCCGAGGACAGCAAGAAGCCGCTGTCCGACGCAAGGATTGCAG
AAGTTTTGGGCCAGCAGGGTATCGTGGTCGCACGACGCACCATCGCGAAGTACCGCGAATCACTGAATATCCCGCCGGTC
AGTCTGCGCAAATCGATCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(331-484)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(122-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6Y4X4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

58.135

100

0.603

Multiple sequence alignment