Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   F543_RS02365 Genome accession   NZ_CP006955
Coordinates   466914..467486 (+) Length   190 a.a.
NCBI ID   WP_015433244.1    Uniprot ID   A0A426FFN3
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-189     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 461914..472486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F543_RS02340 (F543_4860) rpsO 462991..463260 (+) 270 WP_015433249.1 30S ribosomal protein S15 -
  F543_RS02345 (F543_4870) grcA 463388..463771 (-) 384 WP_015433248.1 autonomous glycyl radical cofactor GrcA -
  F543_RS02350 (F543_4880) ung 463888..464550 (+) 663 WP_015433247.1 uracil-DNA glycosylase -
  F543_RS02355 (F543_4890) rmuC 464619..466247 (+) 1629 WP_015433246.1 DNA recombination protein RmuC -
  F543_RS02360 (F543_4900) - 466252..466815 (+) 564 WP_015433245.1 anthranilate synthase component II -
  F543_RS02365 (F543_4910) pilF 466914..467486 (+) 573 WP_015433244.1 tetratricopeptide repeat protein Machinery gene
  F543_RS02370 (F543_4920) - 467541..468527 (+) 987 WP_015433243.1 AEC family transporter -
  F543_RS02375 (F543_4930) - 468509..468907 (-) 399 WP_015433242.1 SufE family protein -
  F543_RS02380 (F543_4940) clpP 469015..469605 (+) 591 WP_025266793.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  F543_RS02385 (F543_4950) clpX 469605..470858 (+) 1254 WP_015433240.1 ATP-dependent protease ATP-binding subunit ClpX -
  F543_RS02390 (F543_4970) lpxK 471028..472017 (+) 990 WP_015433238.1 tetraacyldisaccharide 4'-kinase -
  F543_RS02395 (F543_4980) - 472010..472174 (+) 165 WP_015433237.1 Trm112 family protein -

Sequence

Protein


Download         Length: 190 a.a.        Molecular weight: 21357.36 Da        Isoelectric Point: 7.3201

>NTDB_id=115215 F543_RS02365 WP_015433244.1 466914..467486(+) (pilF) [Bibersteinia trehalosi USDA-ARS-USMARC-189]
MFFAKLPQYLSACLLIFSLSGCVNSQTSADFDRDEALIARASLALAYLEQQDFSKAKENIEKALSHNSQHYLAHLVAAYY
YQQLSDVQHAEQSYQAAIRLSHQIKQNSPLPEVLNNYGTFLCQQGKFVQAYTQFEQALTGEKAYLRQGDTLENMVLCAKQ
ANQLEKMAQALARLALLDAERATKLAKIVK

Nucleotide


Download         Length: 573 bp        

>NTDB_id=115215 F543_RS02365 WP_015433244.1 466914..467486(+) (pilF) [Bibersteinia trehalosi USDA-ARS-USMARC-189]
ATGTTTTTCGCAAAATTGCCTCAATATTTAAGCGCTTGCTTGCTGATTTTTTCACTCAGTGGTTGCGTGAACTCACAAAC
GTCAGCTGATTTTGATAGAGATGAAGCACTAATAGCGCGAGCAAGCCTTGCTCTTGCTTATCTTGAACAGCAGGATTTCT
CAAAAGCGAAAGAGAATATAGAGAAAGCCTTGAGCCACAATAGCCAACATTATTTGGCGCACCTTGTTGCAGCTTATTAC
TATCAGCAACTTAGCGATGTTCAGCATGCAGAGCAAAGTTATCAAGCGGCGATTCGTTTAAGTCATCAGATTAAGCAAAA
TTCACCATTGCCTGAGGTTCTCAATAACTATGGGACATTTTTATGCCAACAAGGTAAATTTGTTCAAGCTTATACGCAAT
TTGAGCAAGCACTTACAGGAGAAAAAGCTTATCTACGCCAAGGTGATACATTAGAAAATATGGTATTGTGTGCCAAGCAG
GCTAACCAGTTAGAGAAAATGGCTCAGGCACTAGCGCGCTTAGCGCTATTAGATGCAGAACGGGCTACAAAACTTGCAAA
AATTGTCAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat-like domain (TPR_21)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A426FFN3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

54.301

97.895

0.532
  pilF/pilF2 Haemophilus influenzae Rd KW20

43.182

92.632

0.4

Multiple sequence alignment