Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   F542_RS02050 Genome accession   NZ_CP006954
Coordinates   422541..423113 (+) Length   190 a.a.
NCBI ID   WP_025266791.1    Uniprot ID   A0A4V7I7Z9
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-188     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417541..428113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F542_RS02025 (F542_4210) rpsO 418618..418887 (+) 270 WP_015433249.1 30S ribosomal protein S15 -
  F542_RS02030 (F542_4220) grcA 419015..419398 (-) 384 WP_015433248.1 autonomous glycyl radical cofactor GrcA -
  F542_RS02035 (F542_4230) ung 419515..420177 (+) 663 WP_015433247.1 uracil-DNA glycosylase -
  F542_RS02040 (F542_4240) rmuC 420246..421874 (+) 1629 WP_025266789.1 DNA recombination protein RmuC -
  F542_RS02045 (F542_4250) - 421879..422442 (+) 564 WP_025266790.1 anthranilate synthase component II -
  F542_RS02050 (F542_4260) pilF 422541..423113 (+) 573 WP_025266791.1 tetratricopeptide repeat protein Machinery gene
  F542_RS02055 (F542_4270) - 423168..424154 (+) 987 WP_025266792.1 AEC family transporter -
  F542_RS02060 (F542_4280) - 424136..424534 (-) 399 WP_015433242.1 SufE family protein -
  F542_RS02065 (F542_4290) clpP 424642..425232 (+) 591 WP_025266793.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  F542_RS02070 (F542_4300) clpX 425232..426485 (+) 1254 WP_025266794.1 ATP-dependent protease ATP-binding subunit ClpX -
  F542_RS02075 (F542_4320) lpxK 426655..427644 (+) 990 WP_025266795.1 tetraacyldisaccharide 4'-kinase -
  F542_RS02080 (F542_4330) - 427637..427801 (+) 165 WP_015433237.1 Trm112 family protein -

Sequence

Protein


Download         Length: 190 a.a.        Molecular weight: 21413.51 Da        Isoelectric Point: 7.3201

>NTDB_id=115190 F542_RS02050 WP_025266791.1 422541..423113(+) (pilF) [Bibersteinia trehalosi USDA-ARS-USMARC-188]
MFFAKLPQYLSACLLIFLFSGCVNLQTSADFDRDEALIARASLALAYLEQQDFSKAKENIEKALSHNSQHYLAHLVAAYY
YQQISDVQHAEQSYQAAIRLSHQIKQNSPLPEVLNNYGTFLCQQGKFVQAYTQFEQALTGEKAYLRQGDTLENMVLCAKQ
ANQLEKMAQALARLALLDAERAAKLAKIVK

Nucleotide


Download         Length: 573 bp        

>NTDB_id=115190 F542_RS02050 WP_025266791.1 422541..423113(+) (pilF) [Bibersteinia trehalosi USDA-ARS-USMARC-188]
ATGTTTTTTGCAAAATTGCCTCAATATTTAAGCGCTTGCTTGCTGATTTTTTTATTCAGCGGTTGCGTAAACCTACAAAC
GTCAGCTGATTTTGATAGAGATGAAGCACTAATAGCGCGAGCAAGCCTTGCTCTTGCTTATCTTGAACAGCAGGATTTCT
CAAAAGCGAAAGAGAATATAGAGAAAGCCTTGAGCCACAATAGCCAACATTATTTGGCGCACCTTGTCGCCGCTTATTAC
TATCAGCAAATTAGCGATGTTCAGCATGCAGAGCAAAGTTATCAGGCAGCGATTCGTTTAAGTCATCAGATTAAGCAAAA
TTCACCATTGCCTGAGGTGCTCAATAACTATGGGACATTTTTATGCCAACAAGGTAAATTTGTTCAAGCTTATACGCAAT
TTGAGCAAGCACTTACAGGAGAAAAAGCTTATCTACGCCAAGGCGATACATTAGAAAATATGGTATTGTGTGCCAAGCAG
GCTAACCAGTTAGAGAAAATGGCTCAGGCACTAGCGCGCTTAGCGCTATTAGATGCAGAACGGGCAGCAAAACTTGCAAA
AATTGTCAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat-like domain (TPR_21)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V7I7Z9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

53.763

97.895

0.526
  pilF/pilF2 Haemophilus influenzae Rd KW20

43.182

92.632

0.4

Multiple sequence alignment