Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   LSO74_RS03815 Genome accession   NZ_OV040719
Coordinates   726202..726741 (-) Length   179 a.a.
NCBI ID   WP_005654843.1    Uniprot ID   A0A0H3PFD4
Organism   Haemophilus influenzae strain 3655 isolate 3655     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721202..731741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO74_RS03785 (KRLU3655_LOCUS713) fdx 721605..721946 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  LSO74_RS03790 (KRLU3655_LOCUS714) iscX 721946..722140 (+) 195 WP_005656371.1 Fe-S cluster assembly protein IscX -
  LSO74_RS03795 (KRLU3655_LOCUS715) - 722191..722805 (-) 615 WP_005656369.1 YfgM family protein -
  LSO74_RS03800 (KRLU3655_LOCUS716) hisS 722823..724094 (-) 1272 WP_005656367.1 histidine--tRNA ligase -
  LSO74_RS03805 (KRLU3655_LOCUS717) ispG 724104..725210 (-) 1107 WP_005656366.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LSO74_RS03810 (KRLU3655_LOCUS718) - 725219..726130 (-) 912 WP_005656363.1 helix-turn-helix domain-containing protein -
  LSO74_RS03815 (KRLU3655_LOCUS719) pilF/pilF2 726202..726741 (-) 540 WP_005654843.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LSO74_RS03820 (KRLU3655_LOCUS720) - 726842..727993 (-) 1152 WP_005656361.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  LSO74_RS03825 (KRLU3655_LOCUS721) - 728240..729118 (+) 879 WP_005656360.1 D-alanyl-D-alanine carboxypeptidase family protein -
  LSO74_RS03830 (KRLU3655_LOCUS722) - 729483..730364 (+) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -
  LSO74_RS03835 (KRLU3655_LOCUS723) - 730364..731284 (+) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20586.20 Da        Isoelectric Point: 8.2626

>NTDB_id=1151771 LSO74_RS03815 WP_005654843.1 726202..726741(-) (pilF/pilF2) [Haemophilus influenzae strain 3655 isolate 3655]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1151771 LSO74_RS03815 WP_005654843.1 726202..726741(-) (pilF/pilF2) [Haemophilus influenzae strain 3655 isolate 3655]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3PFD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment