Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MKZ32_RS15100 Genome accession   NZ_OU912926
Coordinates   3257524..3258951 (-) Length   475 a.a.
NCBI ID   WP_239798023.1    Uniprot ID   -
Organism   Candidatus Nitrotoga arctica strain 6680 isolate 6680     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3252524..3263951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ32_RS15065 (NTG6680_3129) - 3252693..3253292 (-) 600 WP_239798020.1 flavin prenyltransferase UbiX -
  MKZ32_RS15070 (NTG6680_3130) - 3253557..3253961 (-) 405 WP_239795974.1 transposase -
  MKZ32_RS15075 (NTG6680_3132) - 3254167..3254610 (-) 444 WP_239795975.1 helix-turn-helix domain-containing protein -
  MKZ32_RS15080 (NTG6680_3133) rapZ 3254854..3255723 (-) 870 WP_173054204.1 RNase adapter RapZ -
  MKZ32_RS15085 (NTG6680_3134) hprK 3255727..3256665 (-) 939 WP_239798021.1 HPr(Ser) kinase/phosphatase -
  MKZ32_RS15090 (NTG6680_3135) - 3256671..3257126 (-) 456 WP_239288362.1 PTS sugar transporter subunit IIA -
  MKZ32_RS15095 (NTG6680_3136) hpf 3257178..3257507 (-) 330 WP_239798022.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MKZ32_RS15100 (NTG6680_3137) rpoN/rpoN1 3257524..3258951 (-) 1428 WP_239798023.1 RNA polymerase factor sigma-54 Machinery gene
  MKZ32_RS15105 (NTG6680_3138) lptB 3258990..3259712 (-) 723 WP_173054194.1 LPS export ABC transporter ATP-binding protein -
  MKZ32_RS15110 (NTG6680_3139) lptA 3259709..3260281 (-) 573 WP_239798024.1 lipopolysaccharide transport periplasmic protein LptA -
  MKZ32_RS15115 (NTG6680_3140) lptC 3260262..3260855 (-) 594 WP_239798025.1 LPS export ABC transporter periplasmic protein LptC -
  MKZ32_RS15120 (NTG6680_3141) - 3260852..3261370 (-) 519 WP_239798026.1 KdsC family phosphatase -
  MKZ32_RS15125 (NTG6680_3142) - 3261370..3262356 (-) 987 WP_239798027.1 KpsF/GutQ family sugar-phosphate isomerase -
  MKZ32_RS15130 (NTG6680_3143) hisD 3262456..3263754 (-) 1299 WP_239798028.1 histidinol dehydrogenase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 53873.22 Da        Isoelectric Point: 4.8795

>NTDB_id=1151590 MKZ32_RS15100 WP_239798023.1 3257524..3258951(-) (rpoN/rpoN1) [Candidatus Nitrotoga arctica strain 6680 isolate 6680]
MKPTLQLRQSQQLTLTPQLQQAIRLLQLSTLEVNQEVARLLDDNPLLEREDEDAMQPYAHGDAASTAAVDNTEVKDEMPQ
QADEEWVKEDWNESSFSAASGPDDEKEARAEVAADTPSLREHLIWQLNMSQHDVRDRKIISLLIDALDGNGYLAQPLEEI
VEMLPEQLDITLDDLETALVQLQHLDQPGIAARTLSECLALQLKALPEDTPCRDLALRLVSQYLNLLGAHDFAKLKKVLR
CDDDALRTVQSLITHLQPRPGAEFEQRAADYVVPDVVVEFRHGNWHVRLNQGAMPRLRVNQMYANILQQGNEKNVQQLAG
QLQEARWFIKNLQQRFDTILRVSQTIVDRQRNFFEHGDTAMCPLVLREIAEQLDLHESTISRVTTQKFMLTPRGIYELKY
FFCSGIATESGGTCSSTAIRALIKQLVNQEDARHPLTDSRMSGILAQQGILVARRTVAKYRELLNILPVNLRKSL

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=1151590 MKZ32_RS15100 WP_239798023.1 3257524..3258951(-) (rpoN/rpoN1) [Candidatus Nitrotoga arctica strain 6680 isolate 6680]
ATGAAACCCACATTACAACTTAGGCAATCCCAGCAACTGACGCTTACCCCCCAGTTGCAGCAAGCCATACGCCTGTTGCA
ACTATCCACTCTGGAAGTCAACCAGGAAGTGGCACGCTTGCTGGATGATAATCCATTGCTCGAACGCGAAGATGAAGACG
CTATGCAGCCCTATGCACATGGAGATGCTGCCTCTACCGCCGCTGTGGATAATACCGAAGTCAAAGATGAAATGCCGCAA
CAGGCCGATGAAGAATGGGTAAAAGAAGACTGGAATGAATCCTCTTTTTCCGCAGCCAGCGGGCCGGATGATGAAAAAGA
AGCTCGTGCGGAAGTCGCGGCGGATACCCCCAGCCTGCGTGAGCATCTCATCTGGCAACTAAATATGAGCCAGCATGATG
TGCGCGACCGAAAAATTATCAGTCTGTTGATCGACGCATTGGACGGCAACGGTTATCTGGCGCAGCCGTTGGAAGAAATT
GTCGAAATGCTACCAGAACAACTCGACATTACTTTGGATGACTTGGAAACTGCACTGGTGCAATTACAGCACCTGGATCA
GCCAGGCATAGCCGCCCGTACCTTAAGCGAATGTCTGGCGCTACAACTCAAAGCGCTCCCGGAGGATACCCCATGCCGTG
ATCTGGCACTGCGCTTGGTGTCGCAATACCTCAACCTGTTGGGTGCGCACGATTTTGCCAAGCTGAAGAAAGTCCTTCGT
TGTGACGATGACGCATTGCGCACCGTGCAATCGCTCATCACCCATCTGCAACCAAGACCTGGCGCTGAATTTGAACAGCG
AGCTGCAGACTATGTCGTACCTGACGTGGTGGTAGAATTCCGCCACGGTAACTGGCATGTACGACTAAATCAGGGCGCTA
TGCCGCGCCTGCGCGTTAACCAAATGTATGCCAATATCTTGCAACAGGGCAATGAAAAAAACGTACAGCAGCTGGCTGGT
CAATTGCAGGAAGCGCGCTGGTTTATCAAAAATTTGCAACAGCGTTTCGATACTATTTTGCGTGTATCGCAGACAATTGT
TGATCGCCAGCGCAATTTTTTCGAGCACGGCGACACAGCTATGTGCCCGCTGGTACTGCGCGAAATCGCAGAGCAGCTTG
ATCTGCATGAATCCACCATCTCCCGCGTGACTACGCAAAAATTCATGCTCACCCCGCGCGGCATCTATGAACTCAAATAT
TTTTTCTGCAGCGGAATCGCGACCGAAAGTGGCGGTACGTGTTCTTCCACGGCGATCCGCGCACTCATTAAGCAGCTTGT
CAATCAGGAAGACGCACGTCACCCCCTAACCGACAGCCGCATGTCTGGAATCCTGGCACAGCAGGGCATATTGGTTGCCC
GGCGTACCGTGGCAAAATACCGGGAATTGCTGAATATCCTCCCCGTTAATCTTCGTAAATCACTCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-473)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(111-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.499

100

0.533

Multiple sequence alignment