Detailed information NTG1150165

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: DG247_RS02385
• Genome accession: NZ_LT992486
• Coordinates: 475111..475986 (-)
• Length: 291 a.a.
• NCBI ID: WP_000418747.1
• Uniprot ID: H9L4S3
• Organism: Vibrio cholerae strain 4295STDY6534216
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 470111..480986

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DG247_RS02365 (SAMEA4374366_00457)	parC	471061..473346 (+)	2286	WP_000102510.1	DNA topoisomerase IV subunit A	-
DG247_RS02370 (SAMEA4374366_00458)	yacG	473395..473592 (-)	198	WP_000162868.1	DNA gyrase inhibitor YacG	-
DG247_RS02375 (SAMEA4374366_00459)	zapD	473734..474474 (-)	741	WP_000207198.1	cell division protein ZapD	-
DG247_RS02380 (SAMEA4374366_00460)	coaE	474506..475114 (-)	609	WP_000011557.1	dephospho-CoA kinase	-
DG247_RS02385 (SAMEA4374366_00461)	pilD	475111..475986 (-)	876	WP_000418747.1	prepilin peptidase	Machinery gene
DG247_RS02390 (SAMEA4374366_00462)	pilC	476044..477270 (-)	1227	WP_000648511.1	type II secretion system F family protein	Machinery gene
DG247_RS02395 (SAMEA4374366_00463)	pilB	477313..479001 (-)	1689	WP_000957200.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DG247_RS02400 (SAMEA4374366_00464)	pilA	479001..479462 (-)	462	WP_000649326.1	pilin	Machinery gene
DG247_RS02405 (SAMEA4374366_00465)	nadC	479707..480597 (-)	891	WP_000665274.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32433.69 Da
• Isoelectric Point: 7.1618

>NTDB_id=1150165 DG247_RS02385 WP_000418747.1 475111..475986(-) (pilD) [Vibrio cholerae strain 4295STDY6534216]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide

• Length: 876 bp

>NTDB_id=1150165 DG247_RS02385 WP_000418747.1 475111..475986(-) (pilD) [Vibrio cholerae strain 4295STDY6534216]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGTCAAACGCCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTAGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCCACTCACTTTCCGTTCGGAGTTTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTGTTGATTGCGGCAACCTTCATCGACTTCGATACCCTGTTGTTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCTGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCGATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCTCTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: H9L4S3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	100	100	1
pilD	Vibrio campbellii strain DS40M4	75.347	98.969	0.746
pilD	Acinetobacter nosocomialis M2	53.676	93.471	0.502
pilD	Acinetobacter baumannii D1279779	53.309	93.471	0.498
pilD	Neisseria gonorrhoeae MS11	44.792	98.969	0.443