Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   X781_RS02465 Genome accession   NZ_CP006942
Coordinates   495033..495590 (+) Length   185 a.a.
NCBI ID   WP_025235396.1    Uniprot ID   -
Organism   Mannheimia sp. USDA-ARS-USMARC-1261     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 490033..500590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  X781_RS02435 (X781_4740) - 490200..491009 (+) 810 WP_025235392.1 metal ABC transporter permease -
  X781_RS02440 (X781_4750) - 491057..491800 (-) 744 WP_025216715.1 metal ABC transporter ATP-binding protein -
  X781_RS02445 (X781_4760) - 491812..492708 (-) 897 WP_025216716.1 metal ABC transporter substrate-binding protein -
  X781_RS02450 (X781_4770) - 492957..494117 (+) 1161 WP_025235393.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  X781_RS02455 (X781_4780) - 494160..494462 (-) 303 WP_025235394.1 helix-turn-helix transcriptional regulator -
  X781_RS02460 (X781_4790) - 494446..494817 (-) 372 WP_025235395.1 type II toxin-antitoxin system RelE/ParE family toxin -
  X781_RS02465 (X781_4800) pilF 495033..495590 (+) 558 WP_025235396.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  X781_RS02470 (X781_4810) - 495587..497923 (+) 2337 WP_025235397.1 Tex family protein -
  X781_RS02475 (X781_4820) - 498053..498295 (+) 243 WP_025235398.1 hypothetical protein -
  X781_RS02480 (X781_4830) - 498306..498842 (+) 537 WP_144084197.1 DUF805 domain-containing protein -
  X781_RS02485 (X781_4840) - 498826..499377 (+) 552 WP_025235400.1 hypothetical protein -
  X781_RS02490 (X781_4850) - 499392..500213 (-) 822 WP_025235401.1 SDR family NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 21151.90 Da        Isoelectric Point: 6.4670

>NTDB_id=115016 X781_RS02465 WP_025235396.1 495033..495590(+) (pilF) [Mannheimia sp. USDA-ARS-USMARC-1261]
MFAKFLKKITACMAIVLLAACSSQQESSSEPEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDIENAEKAYQQALNLSENRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSPKPYYHQADSLENIILCAKKEPNP
QKVNETLTQLEKLDKARANLLKENQ

Nucleotide


Download         Length: 558 bp        

>NTDB_id=115016 X781_RS02465 WP_025235396.1 495033..495590(+) (pilF) [Mannheimia sp. USDA-ARS-USMARC-1261]
ATGTTTGCAAAATTTCTTAAAAAAATAACCGCTTGTATGGCGATAGTGTTATTGGCGGCGTGTTCTTCTCAGCAAGAATC
TTCTTCCGAACCTGAATTTAATCGCTCTGAAGCAGTGAAAGCCCGCATTAATCTTGCTTTAGCCTATTTGGAACAAAACG
ATTTCCCCAAAGCAAAACAAAATATTGATAAAGCCCTTGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTATTAGCC
TACTATTATCAACAAACAGGTGATATTGAAAATGCTGAAAAAGCCTATCAACAAGCGCTCAATTTAAGTGAAAATCGCCC
TGATGTATTGAATAATTACGGCACTTTTTTGTGTAAACAGGGTCAATTTCAACAGGCGTATCAACAATTTGAACAAGCGG
TAAAAAGCCCAAAACCCTATTATCATCAAGCAGATAGCTTAGAAAATATCATTCTGTGTGCGAAAAAAGAGCCAAACCCG
CAGAAAGTGAATGAAACATTAACCCAGCTCGAAAAATTAGATAAAGCTCGAGCGAATTTATTGAAGGAAAATCAATGA

Domains

Predicted by InterproScan.

MalT-like TPR region (TPR_MalT)

(46-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.636

100

0.643
  pilF/pilF2 Haemophilus influenzae Rd KW20

49.419

92.973

0.459

Multiple sequence alignment