Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   DXE48_RS01420 Genome accession   NZ_LT992473
Coordinates   281478..281975 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus isolate 14_5418     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 276478..286975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE48_RS01380 - 276522..277508 (+) 987 WP_000161315.1 ROK family glucokinase -
  DXE48_RS01385 - 277508..277837 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DXE48_RS01390 - 277834..278457 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DXE48_RS01395 comGA 278509..279483 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  DXE48_RS01400 comGB 279455..280525 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  DXE48_RS01405 comGC 280539..280850 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DXE48_RS01410 comGD 280828..281274 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  DXE48_RS01415 comGE 281261..281560 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  DXE48_RS01420 comGF 281478..281975 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  DXE48_RS01425 - 282072..282218 (+) 147 WP_001789879.1 hypothetical protein -
  DXE48_RS01430 - 282208..282732 (+) 525 WP_001015120.1 shikimate kinase -
  DXE48_RS01435 gcvT 282891..283982 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DXE48_RS01440 gcvPA 284002..285348 (+) 1347 WP_031795510.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  DXE48_RS01445 gcvPB 285341..286813 (+) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=1149945 DXE48_RS01420 WP_001825340.1 281478..281975(+) (comGF) [Staphylococcus aureus isolate 14_5418]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1149945 DXE48_RS01420 WP_001825340.1 281478..281975(+) (comGF) [Staphylococcus aureus isolate 14_5418]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment