Detailed information NTG1148779

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C6H34_RS02770
• Genome accession: NZ_LT963395
• Coordinates: 579423..580295 (+)
• Length: 290 a.a.
• NCBI ID: WP_065349036.1
• Uniprot ID: A0A193SJE1
• Organism: Pseudomonas cerasi isolate PL963
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 574423..585295

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6H34_RS02755	-	575886..576281 (-)	396	WP_083188329.1	pilin	-
C6H34_RS02760 (PL963_00532)	pilB	576507..578201 (+)	1695	WP_046719961.1	type IV-A pilus assembly ATPase PilB	Machinery gene
C6H34_RS02765 (PL963_00533)	pilC	578204..579421 (+)	1218	WP_065349037.1	type II secretion system F family protein	Machinery gene
C6H34_RS02770 (PL963_00534)	pilD	579423..580295 (+)	873	WP_065349036.1	prepilin peptidase	Machinery gene
C6H34_RS02775 (PL963_00535)	coaE	580292..580915 (+)	624	WP_058397527.1	dephospho-CoA kinase	-
C6H34_RS02780 (PL963_00536)	yacG	580912..581121 (+)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
C6H34_RS02785 (PL963_00537)	-	581247..581708 (-)	462	WP_065349035.1	hypothetical protein	-
C6H34_RS02790 (PL963_00538)	-	581834..582463 (+)	630	WP_003345130.1	DUF1780 domain-containing protein	-
C6H34_RS02795 (PL963_00539)	-	582460..582993 (+)	534	WP_065349034.1	MOSC domain-containing protein	-
C6H34_RS31430	-	582929..583114 (+)	186	WP_232002895.1	DUF3094 family protein	-
C6H34_RS02805 (PL963_00540)	-	583210..584508 (+)	1299	WP_003430739.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32001.20 Da
• Isoelectric Point: 8.1507

>NTDB_id=1148779 C6H34_RS02770 WP_065349036.1 579423..580295(+) (pilD) [Pseudomonas cerasi isolate PL963]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPIMMERDWKAQSREMLGLPAEPDQPIFNLNRPHSSCPQCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLGWGLLAMSLIDADHQLLPDSMV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=1148779 C6H34_RS02770 WP_065349036.1 579423..580295(+) (pilD) [Pseudomonas cerasi isolate PL963]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCCCCTCTGGCCTTCGTCACCACCTGCTGCATTCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTATCGCCTGCCAATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAAATGCTCGGCC
TGCCCGCCGAACCCGATCAACCCATCTTCAATCTGAACCGCCCCCATTCCAGCTGCCCGCAATGCGCACACAAAATCCGC
CCGTGGGAGAACCTGCCGGTGATCAGCTATCTGCTGCTGCGTGGTAAATGCTCGCAGTGCAAAGCCCCCATCAGCAAACG
CTACCCATTGGTAGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTGGCCTGGCATTTCGGCTTTGGTTGGCAGGCGGCGG
CGATGCTGGTATTGGGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGCTGTTACCTGATTCTATGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCTTTCGGGCTGTTTACCTCGCTTAACGATGCGCTGTGGGGAGCGGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACTGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCGTTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTCGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A193SJE1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Vibrio cholerae strain A1552	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	51.986	95.517	0.497
pilD	Acinetobacter nosocomialis M2	51.264	95.517	0.49
pilD	Neisseria gonorrhoeae MS11	51.866	92.414	0.479