Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   V529_RS12920 Genome accession   NZ_CP006890
Coordinates   2737927..2738541 (-) Length   204 a.a.
NCBI ID   WP_038459268.1    Uniprot ID   -
Organism   Bacillus velezensis SQR9     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2732927..2743541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V529_RS12895 (V529_26590) rpsT 2733230..2733496 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  V529_RS12900 (V529_26600) holA 2733513..2734554 (-) 1042 Protein_2594 DNA polymerase III subunit delta -
  V529_RS20810 - 2734594..2734745 (-) 152 Protein_2595 hypothetical protein -
  V529_RS20020 (V529_26610) - 2734786..2734920 (+) 135 WP_003152870.1 YqzM family protein -
  V529_RS12910 (V529_26620) comEC 2734939..2737290 (-) 2352 WP_038459264.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  V529_RS12915 (V529_26630) - 2737291..2737860 (-) 570 WP_038459266.1 ComE operon protein 2 -
  V529_RS12920 (V529_26640) comEA 2737927..2738541 (-) 615 WP_038459268.1 helix-hairpin-helix domain-containing protein Machinery gene
  V529_RS12925 (V529_26650) comER 2738600..2739421 (+) 822 WP_012118027.1 late competence protein ComER -
  V529_RS12930 (V529_26660) - 2739490..2740227 (-) 738 WP_038459270.1 class I SAM-dependent methyltransferase -
  V529_RS12935 (V529_26670) rsfS 2740224..2740580 (-) 357 WP_007408260.1 ribosome silencing factor -
  V529_RS12940 (V529_26680) yqeK 2740584..2741159 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  V529_RS12945 (V529_26690) - 2741149..2741718 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  V529_RS12950 (V529_26700) yhbY 2741729..2742019 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -
  V529_RS12955 (V529_26710) aroE 2742013..2742855 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22206.44 Da        Isoelectric Point: 6.4939

>NTDB_id=114853 V529_RS12920 WP_038459268.1 2737927..2738541(-) (comEA) [Bacillus velezensis SQR9]
MTKAMMTEPLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=114853 V529_RS12920 WP_038459268.1 2737927..2738541(-) (comEA) [Bacillus velezensis SQR9]
ATGACGAAAGCGATGATGACGGAGCCGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAAAGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363

Multiple sequence alignment