Detailed information NTG1147951

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: CKV96_RS03280
• Genome accession: NZ_LT906476
• Coordinates: 674045..674494 (-)
• Length: 149 a.a.
• NCBI ID: WP_010946364.1
• Uniprot ID: Q5ZXV4
• Organism: Legionella pneumophila strain NCTC11286
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 669045..679494

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKV96_RS03260 (SAMEA4040027_00631)	-	669290..669682 (-)	393	WP_011945786.1	hypothetical protein	-
CKV96_RS03265 (SAMEA4040027_00632)	-	669819..670196 (+)	378	WP_011945787.1	hypothetical protein	-
CKV96_RS03270 (SAMEA4040027_00633)	-	670397..671704 (+)	1308	WP_011945788.1	tetratricopeptide repeat protein	-
CKV96_RS03275 (SAMEA4040027_00634)	comEC	671760..673967 (-)	2208	WP_011945789.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CKV96_RS03280 (SAMEA4040027_00635)	pilE	674045..674494 (-)	450	WP_010946364.1	type IV pilin protein	Machinery gene
CKV96_RS03285 (SAMEA4040027_00636)	-	674506..678015 (-)	3510	WP_011945790.1	pilus assembly protein	-
CKV96_RS03290 (SAMEA4040027_00637)	-	678028..678540 (-)	513	WP_011945791.1	pilus assembly protein	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 16296.57 Da
• Isoelectric Point: 7.6922

>NTDB_id=1147951 CKV96_RS03280 WP_010946364.1 674045..674494(-) (pilE) [Legionella pneumophila strain NCTC11286]
MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide

• Length: 450 bp

>NTDB_id=1147951 CKV96_RS03280 WP_010946364.1 674045..674494(-) (pilE) [Legionella pneumophila strain NCTC11286]
ATGCTCAGTCATGTACATTTTATGAAGAATAGCCGTATGAAACAATCCGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATACAAAAATCCCGTCGTGCTGATGCTC
ATGCCACATTGACACAAGATCAAATCATTTTAGAACGCTGTTATTCACAGAATTTTTCCTATGCGGCAGCGTGTGGCGCC
CTACCAGCATTTCCTCAAACAACGCCGAATGGGTATTATACTATCAATATTTCAAACCTGACAGCCACAACGTATACCTT
AACCGCAACCCCTGTCGGAACCCAAGCCAAAGATACCGAGTGTGCCAGTATGTCAATTAACCAGGCCAATGTAAAAACAG
CCGTAGATTCCTCCGCTAATGCGCAACCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (12-38)

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (39-124)

3D structure

• AlphaFold DB: Q5ZXV4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Legionella pneumophila str. Paris	100	100	1
pilE	Legionella pneumophila strain ERS1305867	100	100	1