Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   CKV74_RS10220 Genome accession   NZ_LT906463
Coordinates   2164904..2165443 (-) Length   179 a.a.
NCBI ID   WP_039847767.1    Uniprot ID   -
Organism   Haemophilus pittmaniae strain NCTC13334     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2159904..2170443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV74_RS10195 (NCTC13334_02053) - 2160345..2160782 (-) 438 WP_007241534.1 isoprenylcysteine carboxylmethyltransferase family protein -
  CKV74_RS10200 (NCTC13334_02054) - 2160813..2161427 (-) 615 WP_007241845.1 YfgM family protein -
  CKV74_RS10205 (NCTC13334_02055) hisS 2161445..2162716 (-) 1272 WP_095177116.1 histidine--tRNA ligase -
  CKV74_RS10210 (NCTC13334_02056) ispG 2162726..2163832 (-) 1107 WP_095177117.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CKV74_RS10215 (NCTC13334_02057) - 2163844..2164833 (-) 990 WP_007241750.1 RodZ family helix-turn-helix domain-containing protein -
  CKV74_RS10220 (NCTC13334_02058) pilF/pilF2 2164904..2165443 (-) 540 WP_039847767.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CKV74_RS10225 (NCTC13334_02059) - 2165470..2166612 (-) 1143 WP_095177118.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  CKV74_RS10230 (NCTC13334_02060) - 2166918..2167799 (+) 882 WP_007241508.1 metal ABC transporter substrate-binding protein -
  CKV74_RS10235 (NCTC13334_02061) - 2167799..2168695 (+) 897 WP_007241800.1 ATP-binding cassette domain-containing protein -
  CKV74_RS10240 (NCTC13334_02062) - 2168699..2169547 (+) 849 WP_095177119.1 metal ABC transporter permease -
  CKV74_RS10245 (NCTC13334_02063) - 2169549..2170382 (+) 834 WP_095177157.1 metal ABC transporter permease -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20375.94 Da        Isoelectric Point: 7.1027

>NTDB_id=1147725 CKV74_RS10220 WP_039847767.1 2164904..2165443(-) (pilF/pilF2) [Haemophilus pittmaniae strain NCTC13334]
MDLISKKYATIVAVFLLSACVTQHHLDFDKQQAAKARVELALGYLQQNDLVQAKLNLDRALVHAPNYYLVHSALAHFYRL
QGDNENARREFNQALKLDASQGDVHNNYGAFLCAQGEYEKAFEQFNQALISANYYHQTDTLENMTLCAQAAGQQERYQES
FNKLQKMDVARAQKLTQAK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1147725 CKV74_RS10220 WP_039847767.1 2164904..2165443(-) (pilF/pilF2) [Haemophilus pittmaniae strain NCTC13334]
ATGGATTTAATCTCTAAAAAATATGCGACTATCGTAGCTGTATTTTTATTAAGTGCTTGTGTTACTCAACATCATTTGGA
TTTTGATAAGCAACAGGCAGCCAAAGCGCGGGTCGAGCTGGCATTGGGGTATTTGCAGCAAAATGATTTGGTGCAGGCCA
AGCTGAATTTAGATAGAGCTTTGGTTCATGCACCGAATTATTATTTGGTACATTCTGCGTTGGCGCATTTTTACCGCTTG
CAGGGAGATAATGAGAATGCCCGTCGTGAGTTTAATCAAGCCTTGAAATTGGATGCTTCACAGGGTGACGTACATAATAA
TTATGGTGCTTTTTTATGTGCCCAAGGCGAGTATGAAAAAGCCTTTGAGCAATTCAATCAGGCGCTGATTTCCGCCAATT
ATTATCATCAGACGGATACCCTAGAAAATATGACGCTTTGTGCCCAAGCGGCAGGACAGCAGGAACGCTACCAAGAATCT
TTCAATAAGCTGCAAAAGATGGATGTCGCAAGGGCGCAAAAACTGACTCAAGCCAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

53.631

100

0.536
  pilF Glaesserella parasuis strain SC1401

43.429

97.765

0.425

Multiple sequence alignment