Detailed information NTG1147475

Bioinformatically predicted

Overview

• Name: amiF
• Type: Regulator
• Locus tag: CKV85_RS01830
• Genome accession: NZ_LT906454
• Coordinates: 375682..376614 (+)
• Length: 310 a.a.
• NCBI ID: WP_017769011.1
• Uniprot ID: A0A239WJ37
• Organism: Streptococcus acidominimus strain NCTC11291
• Function: internalize XIP (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	377471..383163	375682..376614	flank	857

Gene organization within MGE regions

Location: 375682..383163

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKV85_RS01830 (SAMEA4504048_00383)	amiF	375682..376614 (+)	933	WP_017769011.1	ABC transporter ATP-binding protein	Regulator
CKV85_RS01835	-	376988..378127 (-)	1140	Protein_345	transposase	-
CKV85_RS01840 (SAMEA4504048_00386)	-	378221..379174 (-)	954	WP_095121550.1	IS30 family transposase	-
CKV85_RS01845 (SAMEA4504048_00387)	rpsF	379585..379875 (+)	291	WP_017770045.1	30S ribosomal protein S6	-
CKV85_RS01850 (SAMEA4504048_00388)	ssb	379887..380372 (+)	486	WP_095121702.1	single-stranded DNA-binding protein	Machinery gene
CKV85_RS01855 (SAMEA4504048_00389)	rpsR	380408..380647 (+)	240	WP_000068665.1	30S ribosomal protein S18	-
CKV85_RS01860	-	380779..382109 (+)	1331	Protein_350	IS1182 family transposase	-
CKV85_RS01865 (SAMEA4504048_00392)	-	382159..383163 (+)	1005	WP_095121704.1	IS5 family transposase	-

Sequence

Protein

• Length: 310 a.a.
• Molecular weight: 35080.96 Da
• Isoelectric Point: 6.6979

>NTDB_id=1147475 CKV85_RS01830 WP_017769011.1 375682..376614(+) (amiF) [Streptococcus acidominimus strain NCTC11291]
MENRKKLVELKNVSLTFNKGKSNEVKAINNISFDIYEGEVFGLVGESGSGKTTVGRAILKLYDINEGEIIFNGEKVSHLK
GKAQHDFRKQAQMIFQDPQASLNGRMKIRDIVAEGLDIHKLVSSKAERDEKVQHLLDLVGLNKDHLTRYPHEFSGGQRQR
IGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQHEQGLTYLFIAHDLSMVKYISDRIGVMHWGKMLEIGTSEDV
YNNPIHPYTKSLLSAIPEPDPEKERTRVHQMYDPSAELDGQERTMHEITPGHFVLATEAEAEQYRKEARN

Nucleotide

• Length: 933 bp

>NTDB_id=1147475 CKV85_RS01830 WP_017769011.1 375682..376614(+) (amiF) [Streptococcus acidominimus strain NCTC11291]
GTGGAAAACAGGAAAAAATTAGTTGAATTAAAAAATGTTTCCCTAACCTTCAATAAAGGAAAATCAAACGAAGTAAAAGC
CATCAACAATATTAGTTTTGATATTTATGAAGGCGAAGTATTTGGTTTGGTTGGGGAGTCTGGTTCAGGAAAGACAACTG
TCGGACGTGCTATTTTAAAACTTTATGATATCAATGAAGGTGAGATTATTTTTAATGGTGAGAAGGTATCTCATCTTAAA
GGTAAGGCACAACATGACTTCCGAAAACAGGCTCAAATGATTTTCCAGGATCCTCAAGCCAGTTTGAATGGCCGTATGAA
AATTAGGGATATTGTTGCCGAAGGTTTGGATATCCACAAATTGGTGTCTTCAAAGGCTGAACGTGATGAAAAGGTACAGC
ATTTACTTGATTTAGTTGGTTTGAATAAAGACCATTTGACGCGGTATCCGCATGAGTTTTCAGGTGGTCAAAGACAACGG
ATTGGTATTGCTCGTGCCTTGGCTGTAGAACCTAAATTTATCATTGCCGATGAACCGATTTCAGCATTGGATGTATCTAT
CCAAGCTCAAGTTGTGAATTTGATGCAAAAATTACAGCATGAGCAAGGATTGACATATCTTTTCATTGCACATGACCTCT
CTATGGTGAAATACATTTCTGATCGTATAGGGGTTATGCATTGGGGTAAAATGTTGGAAATTGGAACATCTGAAGATGTC
TATAATAATCCCATTCATCCTTATACCAAGAGCTTGCTATCAGCGATTCCAGAACCAGATCCTGAGAAAGAGCGTACCCG
TGTCCATCAGATGTACGATCCATCTGCAGAACTTGATGGTCAAGAACGCACTATGCATGAGATTACTCCAGGACATTTTG
TACTTGCTACAGAAGCGGAAGCAGAGCAATATCGAAAAGAAGCTAGAAATTAG

Domains

Predicted by InterproScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (232-267)

• ABC transporter (ABC_tran) (29-180)

3D structure

• AlphaFold DB: A0A239WJ37

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiF	Streptococcus thermophilus LMD-9	55.41	98.387	0.545
amiF	Streptococcus salivarius strain HSISS4	55.41	98.387	0.545
amiF	Streptococcus thermophilus LMG 18311	55.082	98.387	0.542