Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   CKV78_RS06720 Genome accession   NZ_LT906448
Coordinates   1474345..1474890 (-) Length   181 a.a.
NCBI ID   WP_005763205.1    Uniprot ID   C9PNC5
Organism   Pasteurella dagmatis strain NCTC11617     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1469345..1479890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV78_RS06700 (SAMEA4362423_01376) - 1470074..1470688 (-) 615 WP_005763196.1 YfgM family protein -
  CKV78_RS06705 (SAMEA4362423_01377) hisS 1470706..1471977 (-) 1272 WP_005763198.1 histidine--tRNA ligase -
  CKV78_RS06710 (SAMEA4362423_01378) ispG 1472008..1473111 (-) 1104 WP_005763200.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CKV78_RS06715 (SAMEA4362423_01379) - 1473139..1474224 (-) 1086 WP_032855339.1 RodZ family helix-turn-helix domain-containing protein -
  CKV78_RS06720 (SAMEA4362423_01380) pilF/pilF2 1474345..1474890 (-) 546 WP_005763205.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CKV78_RS06725 (SAMEA4362423_01381) - 1474925..1476100 (-) 1176 WP_005763207.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  CKV78_RS06730 (SAMEA4362423_01382) - 1476342..1476866 (-) 525 WP_032855341.1 HXXEE domain-containing protein -
  CKV78_RS06735 (SAMEA4362423_01383) - 1476992..1478245 (-) 1254 WP_005763210.1 AI-2E family transporter -
  CKV78_RS06740 (SAMEA4362423_01384) glnB 1478246..1478584 (-) 339 WP_005763212.1 nitrogen regulatory protein P-II -
  CKV78_RS06745 (SAMEA4362423_01385) mog 1478611..1479198 (-) 588 WP_005763214.1 molybdopterin adenylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20505.28 Da        Isoelectric Point: 8.7280

>NTDB_id=1147366 CKV78_RS06720 WP_005763205.1 1474345..1474890(-) (pilF/pilF2) [Pasteurella dagmatis strain NCTC11617]
MNRTLKLWIASIFSLFLCACVSQSEKQTVNLEQAAKARVELGLGYLAQNDFVQSKKNLDKALAHSPNSYLVHSALAYFYQ
KQGDAENATNAYLTAIKLSPQQGDVLNNYGAFLCSQGKFEQAYAHFEQALQSKNYYQQADTYENMALCAFAQRNTEIYQH
AVQKLMNIDESRVRKLPKTLK

Nucleotide


Download         Length: 546 bp        

>NTDB_id=1147366 CKV78_RS06720 WP_005763205.1 1474345..1474890(-) (pilF/pilF2) [Pasteurella dagmatis strain NCTC11617]
ATGAACCGCACTTTGAAATTATGGATAGCGAGCATTTTTTCTCTTTTTCTTTGTGCTTGTGTTTCACAATCAGAAAAACA
GACTGTGAATTTAGAGCAAGCTGCAAAAGCGAGAGTGGAATTAGGTTTAGGTTACTTAGCTCAAAATGATTTTGTGCAAT
CTAAGAAAAACCTTGATAAAGCTCTTGCGCATTCACCAAATTCTTATTTAGTTCATTCAGCATTAGCTTATTTTTATCAA
AAACAAGGTGATGCTGAAAATGCCACTAATGCTTATTTAACTGCGATTAAACTGAGCCCACAGCAAGGAGATGTGTTAAA
TAATTACGGTGCATTTTTGTGTAGTCAAGGCAAGTTTGAACAAGCGTATGCACATTTTGAACAAGCGTTACAAAGTAAAA
ATTATTATCAGCAAGCAGATACTTATGAAAATATGGCTCTTTGTGCTTTTGCACAAAGAAATACAGAAATTTATCAACAC
GCTGTGCAGAAATTGATGAATATTGATGAAAGCCGAGTCAGAAAACTTCCTAAAACCTTAAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C9PNC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

53.977

97.238

0.525
  pilF Glaesserella parasuis strain SC1401

47.953

94.475

0.453

Multiple sequence alignment