Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   CKV83_RS01070 Genome accession   NZ_LT906439
Coordinates   233279..234583 (+) Length   434 a.a.
NCBI ID   WP_018373114.1    Uniprot ID   A0A239SLV0
Organism   Streptococcus merionis strain NCTC13788     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 228279..239583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV83_RS01050 (SAMEA4412692_00214) - 229381..230781 (+) 1401 WP_018373110.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  CKV83_RS01055 (SAMEA4412692_00215) - 230781..231146 (+) 366 WP_018373111.1 S1 RNA-binding domain-containing protein -
  CKV83_RS01060 (SAMEA4412692_00216) cysK 231389..232312 (-) 924 WP_018373112.1 cysteine synthase A -
  CKV83_RS01065 (SAMEA4412692_00217) - 232594..233223 (-) 630 WP_018373113.1 YigZ family protein -
  CKV83_RS01070 (SAMEA4412692_00218) comFA/cflA 233279..234583 (+) 1305 WP_018373114.1 DEAD/DEAH box helicase Machinery gene
  CKV83_RS01075 (SAMEA4412692_00219) - 234691..235245 (+) 555 WP_231869669.1 ComF family protein -
  CKV83_RS01080 (SAMEA4412692_00220) hpf 235326..235865 (+) 540 WP_018373116.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CKV83_RS01085 (SAMEA4412692_00221) - 236284..237615 (+) 1332 WP_018373117.1 hemolysin family protein -
  CKV83_RS01090 - 238042..238242 (+) 201 WP_018373118.1 cold-shock protein -
  CKV83_RS01095 (SAMEA4412692_00223) pflA 238387..239181 (+) 795 WP_026216892.1 pyruvate formate-lyase-activating protein -

Sequence

Protein


Download         Length: 434 a.a.        Molecular weight: 49322.90 Da        Isoelectric Point: 7.9610

>NTDB_id=1147122 CKV83_RS01070 WP_018373114.1 233279..234583(+) (comFA/cflA) [Streptococcus merionis strain NCTC13788]
METLDDYKGRLLTKEQVPEELLPYARQLPAVRQEKGKHFCERCGSELDQEKNRLPMGAIYCRECLILGRVRSDQPLYYFP
QAPFEPQCTLKWQGQLTKWQQGISEGLCENTRQRKNTLVHAVTGAGKTEMIYQVIADHIAEGKAVCIATPRIDVCIELYR
RLSQDFSCPIALLHGESEPYFRTPLVIATTHQLLKFYRAFDLLIIDEVDAFPFVDNATLYHAVDQCISENGCKIFLTATT
TDALEKQVRSGELSLLRLPRRFHENPLVVPKKVWLNSFMKYLSKNKLPAKLITDITNQRQTGHPLLIFAPEIATGQHLVK
VLKNDFPQEQIGFVSSQTENRLEIVTAFRDQELTILVSTTILERGVTFPGVDVFVVEANHRLYTKSSLVQIGGRVGRSSE
RPTGLLNFYQNGTNRAIEKAIVEIKLMNKESGLV

Nucleotide


Download         Length: 1305 bp        

>NTDB_id=1147122 CKV83_RS01070 WP_018373114.1 233279..234583(+) (comFA/cflA) [Streptococcus merionis strain NCTC13788]
ATGGAAACATTGGATGATTACAAAGGAAGGTTGTTGACAAAAGAGCAAGTTCCAGAAGAATTATTGCCCTATGCAAGACA
ATTACCTGCTGTGAGACAAGAGAAAGGAAAACACTTTTGTGAGCGTTGTGGTAGCGAGCTTGATCAAGAAAAAAATCGCC
TGCCAATGGGAGCGATCTATTGCAGGGAGTGTCTCATTCTGGGGCGAGTTCGCAGTGATCAGCCGCTTTATTATTTTCCT
CAAGCTCCCTTTGAACCTCAGTGTACTCTGAAATGGCAGGGCCAGTTGACTAAATGGCAACAGGGAATCTCAGAAGGGTT
GTGCGAAAACACACGACAGAGAAAAAATACTTTAGTACACGCTGTTACGGGTGCAGGTAAGACAGAAATGATCTATCAGG
TGATTGCTGATCATATCGCTGAGGGAAAGGCTGTTTGTATTGCTACGCCAAGGATTGACGTCTGTATTGAACTCTATCGG
AGACTTTCTCAAGATTTCAGCTGCCCCATTGCCCTTCTACATGGTGAGAGCGAGCCTTATTTTCGAACACCCCTTGTCAT
TGCGACGACTCACCAATTGCTCAAGTTTTACCGCGCTTTTGACTTGCTTATCATTGATGAAGTAGATGCCTTTCCTTTTG
TGGACAATGCTACGCTTTACCATGCAGTGGATCAATGTATTTCAGAAAATGGGTGCAAAATTTTTCTGACAGCAACCACG
ACAGATGCCTTGGAGAAGCAAGTGAGGTCAGGAGAACTCAGTTTACTGCGATTACCAAGGCGTTTTCATGAAAATCCGCT
GGTTGTTCCAAAGAAGGTCTGGCTCAACTCTTTTATGAAATACCTTTCTAAAAACAAATTACCAGCCAAACTGATAACAG
ACATCACCAATCAGCGTCAGACAGGCCATCCGTTGCTTATCTTTGCGCCAGAGATTGCTACAGGGCAGCATCTCGTTAAA
GTCTTGAAAAACGACTTCCCTCAAGAACAAATCGGTTTTGTCTCTAGCCAAACCGAAAATCGCTTGGAAATCGTGACAGC
TTTTCGGGATCAAGAACTAACTATTTTAGTATCAACAACTATTTTAGAGCGAGGAGTAACCTTTCCAGGAGTTGATGTCT
TTGTGGTTGAGGCCAATCATCGTCTTTACACAAAATCGAGTCTAGTTCAGATTGGCGGACGAGTCGGCAGGAGTTCTGAA
CGACCGACAGGGCTTCTTAATTTTTACCAAAATGGAACTAACCGCGCTATTGAAAAAGCAATTGTTGAAATAAAGCTCAT
GAATAAGGAGAGTGGCCTCGTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(292-398)

DEAD/DEAH box helicase (DEAD)

(110-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A239SLV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

66.589

98.618

0.657
  comFA/cflA Streptococcus pneumoniae D39

66.589

98.618

0.657
  comFA/cflA Streptococcus pneumoniae R6

66.589

98.618

0.657
  comFA/cflA Streptococcus pneumoniae TIGR4

66.589

98.618

0.657
  comFA/cflA Streptococcus mitis NCTC 12261

64.434

99.77

0.643
  comFA/cflA Streptococcus mitis SK321

64.203

99.77

0.641
  comFA Lactococcus lactis subsp. cremoris KW2

50.254

90.783

0.456
  comFA Latilactobacillus sakei subsp. sakei 23K

39.32

94.931

0.373

Multiple sequence alignment