Detailed information NTG1145492

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BT141_RS07810
• Genome accession: NZ_LT632614
• Coordinates: 1704691..1705554 (+)
• Length: 287 a.a.
• NCBI ID: WP_011946525.1
• Uniprot ID: I7HJU8
• Organism: Legionella pneumophila strain ST62 isolate ST62
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1699691..1710554

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BT141_RS07790 (H044120014_01542)	-	1700184..1700510 (-)	327	WP_011213802.1	DUF1820 family protein	-
BT141_RS07795 (H044120014_01543)	-	1700693..1701436 (-)	744	WP_011213803.1	methyltransferase domain-containing protein	-
BT141_RS07800 (H044120014_01544)	pilB	1701695..1703419 (+)	1725	WP_011946523.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BT141_RS07805 (H044120014_01545)	pilC	1703425..1704645 (+)	1221	WP_027226540.1	type II secretion system F family protein	Machinery gene
BT141_RS07810 (H044120014_01546)	pilD	1704691..1705554 (+)	864	WP_011946525.1	prepilin peptidase	Machinery gene
BT141_RS07815 (H044120014_01547)	-	1705568..1706773 (-)	1206	WP_061363796.1	cyclic nucleotide-binding domain-containing protein	-
BT141_RS07820 (H044120014_01548)	-	1706881..1707474 (-)	594	WP_011213808.1	hypothetical protein	-
BT141_RS07825 (H044120014_01549)	-	1707490..1707945 (-)	456	WP_042233877.1	hypothetical protein	-
BT141_RS07830 (H044120014_01550)	-	1708079..1708894 (+)	816	WP_011215545.1	pyruvate, water dikinase regulatory protein	-
BT141_RS07835 (H044120014_01551)	-	1709015..1710463 (-)	1449	WP_042233874.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32800.45 Da
• Isoelectric Point: 7.8327

>NTDB_id=1145492 BT141_RS07810 WP_011946525.1 1704691..1705554(+) (pilD) [Legionella pneumophila strain ST62 isolate ST62]
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFQLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNIFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDLIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=1145492 BT141_RS07810 WP_011946525.1 1704691..1705554(+) (pilD) [Legionella pneumophila strain ST62 isolate ST62]
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCTGTTGGAAGTTT
GCTTAATGTGATAATCTACCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCAGCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGCCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGCGATAGTCCAATTTCAATTCGCTA
CCCTTTTGTTGAGACTCTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAACTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTCATTGCTAATACCCAGAATATATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCCTATTTAGCCTTATGGTTATTCATTAATCTGTTTTATCTGATGACAGGTAAAGTAGGTATGGGACATGGGGATTTCA
AATTATTTGCAGCATTTGGTGCATGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACCGGGGCT
ATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGATTTAATTATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

3D structure

• AlphaFold DB: I7HJU8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.11	97.909	0.481
pilD	Acinetobacter nosocomialis M2	48.352	95.122	0.46
pilD	Acinetobacter baumannii D1279779	47.636	95.819	0.456
pilD	Vibrio campbellii strain DS40M4	45.683	96.864	0.443
pilD	Neisseria gonorrhoeae MS11	42.446	96.864	0.411