Detailed information NTG1145348

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: DAQ1742_RS05725
• Genome accession: NZ_LT615367
• Coordinates: 1252941..1253792 (+)
• Length: 283 a.a.
• NCBI ID: WP_180706251.1
• Uniprot ID: A0A375A8L3
• Organism: Dickeya aquatica strain 174/2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1247941..1258792

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DAQ1742_RS05695 (DAQ1742_01245)	gspH	1248361..1248897 (+)	537	WP_067487193.1	type II secretion system minor pseudopilin GspH	-
DAQ1742_RS05700 (DAQ1742_01246)	gspI	1248911..1249288 (+)	378	WP_035343324.1	type II secretion system minor pseudopilin GspI	-
DAQ1742_RS05705 (DAQ1742_01247)	gspJ	1249285..1250028 (+)	744	WP_074384649.1	type II secretion system minor pseudopilin GspJ	-
DAQ1742_RS05710 (DAQ1742_01248)	gspK	1250025..1251092 (+)	1068	WP_035343320.1	type II secretion system minor pseudopilin GspK	-
DAQ1742_RS05715 (DAQ1742_01249)	gspL	1251089..1252291 (+)	1203	WP_067487186.1	type II secretion system protein GspL	-
DAQ1742_RS05720 (DAQ1742_01250)	gspM	1252288..1252776 (+)	489	WP_035343315.1	type II secretion system protein GspM	-
DAQ1742_RS05725 (DAQ1742_01251)	pilD	1252941..1253792 (+)	852	WP_180706251.1	A24 family peptidase	Machinery gene
DAQ1742_RS20840	ypdK	1253978..1254037 (-)	60	WP_099048981.1	membrane protein YpdK	-
DAQ1742_RS05735 (DAQ1742_01253)	-	1254536..1255111 (+)	576	WP_035343311.1	alpha/beta hydrolase	-
DAQ1742_RS05740 (DAQ1742_01255)	-	1255283..1255723 (-)	441	WP_035343310.1	hotdog fold thioesterase	-
DAQ1742_RS05745 (DAQ1742_01256)	dhbA	1255723..1256481 (-)	759	WP_180706252.1	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase	-
DAQ1742_RS05750 (DAQ1742_01257)	-	1256478..1257350 (-)	873	WP_035343306.1	isochorismatase	-

Sequence

Protein

• Length: 283 a.a.
• Molecular weight: 31279.25 Da
• Isoelectric Point: 9.2413

>NTDB_id=1145348 DAQ1742_RS05725 WP_180706251.1 1252941..1253792(+) (pilD) [Dickeya aquatica strain 174/2]
MDLLAFASAFPRVWLATLLLLGVMIGSFLNVVIYRLPQMLERRWQQEARFHLNMPAGVPASRYDLIWPPSSCPHCSKRLR
VIDNIPLLSWLWLRGRAHCCGGEVSWRYPLTELLTGALFMLAGALWQPGFALLGASVCFSLLLVLATIDARTQLLPDVLT
FPLLWLGLAFNLSDTFVPLSEAVIGALAGYLSLWLIYWAFRLLSGREALGHGDFKLLSALGAWVGWQALPNLVLIASLAG
LVITLVWRSVRRMNMQQPLAFGPWLALAGAIALILNALGNGSY

Nucleotide

• Length: 852 bp

>NTDB_id=1145348 DAQ1742_RS05725 WP_180706251.1 1252941..1253792(+) (pilD) [Dickeya aquatica strain 174/2]
ATGGATCTGCTCGCCTTCGCCAGCGCGTTTCCGCGCGTTTGGCTGGCAACCTTGCTGTTGCTGGGGGTGATGATTGGCAG
CTTTTTGAATGTGGTTATTTATCGTTTACCACAGATGCTGGAGCGCCGCTGGCAACAGGAGGCGCGTTTCCACCTGAATA
TGCCAGCGGGTGTTCCCGCCAGCCGTTATGATCTTATCTGGCCGCCCTCATCCTGCCCGCACTGCTCTAAGCGTTTGCGG
GTGATTGATAATATCCCCTTACTGAGCTGGTTATGGCTGCGTGGGCGTGCGCACTGCTGTGGTGGCGAGGTGTCGTGGCG
CTATCCGCTGACGGAACTGCTCACCGGTGCGCTGTTTATGCTGGCAGGGGCGCTCTGGCAACCAGGGTTTGCGCTACTGG
GGGCCTCCGTGTGTTTCAGCTTGCTGTTAGTGCTGGCGACTATTGATGCCCGCACTCAACTGCTACCGGATGTCTTGACG
TTTCCCCTGCTATGGCTTGGCCTGGCGTTTAACCTGTCTGATACCTTTGTCCCGCTCAGTGAGGCGGTGATCGGCGCACT
GGCAGGCTATCTGTCGCTATGGTTGATTTACTGGGCGTTTCGCCTGCTGAGTGGCCGGGAGGCATTAGGACATGGTGATT
TTAAACTGCTGTCAGCGCTAGGTGCGTGGGTGGGGTGGCAGGCGCTCCCCAATCTGGTGTTGATAGCGTCCTTGGCCGGG
CTGGTGATAACGCTGGTATGGCGCAGTGTTCGCCGCATGAATATGCAACAGCCGCTGGCGTTTGGCCCCTGGCTGGCACT
GGCAGGAGCGATAGCGCTGATACTGAATGCCTTGGGCAACGGCAGCTATTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-122)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: A0A375A8L3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.754	99.293	0.484
pilD	Neisseria gonorrhoeae MS11	48.846	91.873	0.449
pilD	Vibrio campbellii strain DS40M4	43.494	95.053	0.413
pilD	Acinetobacter baumannii D1279779	43.75	90.459	0.396
pilD	Acinetobacter nosocomialis M2	43.75	90.459	0.396