Detailed information NTG1145171

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: B5474_RS20440
• Genome accession: NZ_LT605059
• Coordinates: 349053..349913 (-)
• Length: 286 a.a.
• NCBI ID: WP_001152277.1
• Uniprot ID: A0A009HWK1
• Organism: Acinetobacter baumannii strain NCTC7364
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 344053..354913

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B5474_RS01640 (NCTC7364_00330)	-	344944..346254 (+)	1311	WP_079548021.1	tetratricopeptide repeat protein	-
B5474_RS01645 (NCTC7364_00331)	-	346331..346657 (+)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
B5474_RS01650 (NCTC7364_00332)	rlmB	346759..347508 (+)	750	WP_001118149.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
B5474_RS01655 (NCTC7364_00333)	-	347544..348458 (+)	915	WP_079548022.1	DMT family transporter	-
B5474_RS01660 (NCTC7364_00334)	coaE	348455..349051 (-)	597	WP_031379557.1	dephospho-CoA kinase	-
B5474_RS20440 (NCTC7364_00335)	pilD	349053..349913 (-)	861	WP_001152277.1	prepilin peptidase	Machinery gene
B5474_RS01670 (NCTC7364_00336)	pilC	349913..351139 (-)	1227	WP_000279216.1	type II secretion system F family protein	Machinery gene
B5474_RS01675 (NCTC7364_00337)	pilB	351169..352881 (-)	1713	WP_001274987.1	type IV-A pilus assembly ATPase PilB	Machinery gene
B5474_RS01680 (NCTC7364_00338)	tpiA	353172..353966 (+)	795	WP_000016939.1	triose-phosphate isomerase	-
B5474_RS01685 (NCTC7364_00339)	secG	353979..354308 (+)	330	WP_000555570.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32038.43 Da
• Isoelectric Point: 7.1041

>NTDB_id=1145171 B5474_RS20440 WP_001152277.1 349053..349913(-) (pilD) [Acinetobacter baumannii strain NCTC7364]
MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACQQP
IRWYQNIPVISWLVLRGKCGHCQHPISIRYPAIELLTMLCSLVVVIVFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=1145171 B5474_RS20440 WP_001152277.1 349053..349913(-) (pilD) [Acinetobacter baumannii strain NCTC7364]
ATGCAAGACATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTTAATGTAGTGATTTACCGCACGCCAAAAATGATGGAGCAAGATTGGCAGCAAGAATGCCAAATGCTGCTCA
ATCCTGAGCAACCCATTATTGATCATGAGAAGTTAACTTTAAGTAAGCCTGCTTCATCGTGCCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCTGTTATAAGCTGGCTTGTGTTAAGAGGAAAATGTGGTCATTGCCAGCACCCGATTAG
TATTCGCTATCCAGCCATCGAACTACTCACTATGTTATGTTCATTAGTAGTCGTCATAGTGTTTGGTCCAACCATACAAA
TGCTTTTTGGACTCGTCCTCACATGGGTACTGATAGCCCTTACCTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACCTTACCTTTAGCTGCACTCGGCTTAGGTATTAATACCTTTAATATTTATACCTCACCCAACTCAGCCATTTGGGG
TTATCTCATTGGTTTCCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTGATCACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGTGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCATCGTTA
CTTGGCGCAATCATTGGCATCATTTTATTAAAATTAAGAAATGACAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCTGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-127)

3D structure

• AlphaFold DB: A0A009HWK1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	98.951	100	0.99
pilD	Acinetobacter nosocomialis M2	98.601	100	0.986
pilD	Vibrio cholerae strain A1552	54.044	95.105	0.514
pilD	Vibrio campbellii strain DS40M4	52.768	94.755	0.5
pilD	Neisseria gonorrhoeae MS11	47.727	92.308	0.441