Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BN8422_RS08410 Genome accession   NZ_LT598688
Coordinates   1660992..1661678 (-) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus isolate Sa_Newman_UoM     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1655992..1666678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN8422_RS08390 rpsT 1656906..1657157 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  BN8422_RS08395 holA 1657202..1658176 (-) 975 WP_001282562.1 DNA polymerase III subunit delta -
  BN8422_RS08400 comEC 1658233..1660434 (-) 2202 WP_001566051.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BN8422_RS08405 - 1660439..1660900 (-) 462 WP_000439693.1 ComE operon protein 2 -
  BN8422_RS08410 comEA 1660992..1661678 (-) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  BN8422_RS08415 - 1661718..1662434 (-) 717 WP_000084829.1 class I SAM-dependent DNA methyltransferase -
  BN8422_RS08420 rsfS 1662437..1662790 (-) 354 WP_001088022.1 ribosome silencing factor -
  BN8422_RS08425 yqeK 1662791..1663375 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BN8422_RS08430 nadD 1663365..1663934 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  BN8422_RS08435 yhbY 1663937..1664227 (-) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  BN8422_RS08440 aroE 1664231..1665037 (-) 807 WP_000666761.1 shikimate dehydrogenase -
  BN8422_RS08445 yqeH 1665051..1666151 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=1144829 BN8422_RS08410 WP_001792568.1 1660992..1661678(-) (comEA) [Staphylococcus aureus isolate Sa_Newman_UoM]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=1144829 BN8422_RS08410 WP_001792568.1 1660992..1661678(-) (comEA) [Staphylococcus aureus isolate Sa_Newman_UoM]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969

Multiple sequence alignment