Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   BN8422_RS08155 Genome accession   NZ_LT598688
Coordinates   1613624..1614121 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus isolate Sa_Newman_UoM     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1608624..1619121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN8422_RS08130 gcvPB 1608786..1610258 (-) 1473 WP_000202192.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  BN8422_RS08135 gcvPA 1610251..1611597 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  BN8422_RS08140 gcvT 1611617..1612708 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  BN8422_RS08145 - 1612867..1613391 (-) 525 WP_001015117.1 shikimate kinase -
  BN8422_RS08150 - 1613381..1613527 (-) 147 WP_001789879.1 hypothetical protein -
  BN8422_RS08155 comGF 1613624..1614121 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  BN8422_RS08160 comGE 1614039..1614338 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  BN8422_RS08165 comGD 1614325..1614771 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  BN8422_RS08170 comGC 1614749..1615060 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  BN8422_RS08175 comGB 1615074..1616144 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  BN8422_RS08180 comGA 1616116..1617090 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  BN8422_RS08185 - 1617142..1617765 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  BN8422_RS08190 - 1617762..1618091 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  BN8422_RS08195 - 1618091..1619077 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=1144821 BN8422_RS08155 WP_001789864.1 1613624..1614121(-) (comGF) [Staphylococcus aureus isolate Sa_Newman_UoM]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1144821 BN8422_RS08155 WP_001789864.1 1613624..1614121(-) (comGF) [Staphylococcus aureus isolate Sa_Newman_UoM]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment