Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NZAK3_RS08030 Genome accession   NZ_LT009690
Coordinates   1622766..1623263 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NZAK3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1617766..1628263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NZAK3_RS08005 gcvPB 1617928..1619400 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  NZAK3_RS08010 gcvPA 1619393..1620739 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  NZAK3_RS08015 gcvT 1620759..1621850 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  NZAK3_RS08020 - 1622009..1622533 (-) 525 WP_001015120.1 shikimate kinase -
  NZAK3_RS08025 - 1622523..1622669 (-) 147 WP_001792109.1 hypothetical protein -
  NZAK3_RS08030 comGF 1622766..1623263 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  NZAK3_RS08035 comGE 1623181..1623480 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  NZAK3_RS08040 comGD 1623467..1623913 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  NZAK3_RS08045 comGC 1623891..1624202 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  NZAK3_RS08050 comGB 1624216..1625286 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  NZAK3_RS08055 comGA 1625258..1626232 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NZAK3_RS08060 - 1626284..1626907 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NZAK3_RS08065 - 1626904..1627233 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NZAK3_RS08070 - 1627233..1628219 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=1143574 NZAK3_RS08030 WP_001788897.1 1622766..1623263(-) (comGF) [Staphylococcus aureus strain NZAK3]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1143574 NZAK3_RS08030 WP_001788897.1 1622766..1623263(-) (comGF) [Staphylococcus aureus strain NZAK3]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment