Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   STN4L_RS05390 Genome accession   NZ_LS974444
Coordinates   1035700..1037019 (-) Length   439 a.a.
NCBI ID   WP_011680770.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain N4L     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1026310..1035719 1035700..1037019 flank -19


Gene organization within MGE regions


Location: 1026310..1037019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STN4L_RS05315 (STN4L_01236) - 1026310..1026843 (+) 534 WP_011680775.1 DUF402 domain-containing protein -
  STN4L_RS05320 (STN4L_01237) - 1026873..1027532 (-) 660 WP_011225520.1 DUF1803 domain-containing protein -
  STN4L_RS05325 (STN4L_01238) - 1027586..1028518 (-) 933 WP_011680773.1 manganese-dependent inorganic pyrophosphatase -
  STN4L_RS05380 (STN4L_01249) hpf 1034430..1034978 (-) 549 WP_011680772.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  STN4L_RS05385 (STN4L_01250) - 1035057..1035719 (-) 663 WP_011680771.1 ComF family protein -
  STN4L_RS05390 (STN4L_01251) comFA/cflA 1035700..1037019 (-) 1320 WP_011680770.1 DEAD/DEAH box helicase Machinery gene

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50615.77 Da        Isoelectric Point: 9.7105

>NTDB_id=1143032 STN4L_RS05390 WP_011680770.1 1035700..1037019(-) (comFA/cflA) [Streptococcus thermophilus strain N4L]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVTGAGKTEMIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKHHLASRFHGNPLVLPRFFWQGRLQKSLTSEKLPRPLIHQIKKQRKSNFPLLIFFPNIALGEKFSIT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELTILVTTTILERGVTFPQVDVFVCMANHHLYTSSSLIQIGGRVGRSPER
PTGKLYFFHEGLSKSMLQCRKEINAMNKKGGFENEVSTM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=1143032 STN4L_RS05390 WP_011680770.1 1035700..1037019(-) (comFA/cflA) [Streptococcus thermophilus strain N4L]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGATTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGACTGGTGCTGGCAAGACAGAGATGATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGACGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTGACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGCATCACCTTGCTAGTCGTTTCCACGGAAATCCTCTAGT
CCTTCCTCGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAGCGAGAAGCTTCCAAGGCCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTCCCCAATATAGCATTAGGTGAAAAGTTTAGTATTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGACAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATCACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTACAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGAAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-396)

Type III restriction enzyme, res subunit (ResIII)

(94-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae TIGR4

55.452

98.178

0.544
  comFA/cflA Streptococcus pneumoniae Rx1

55.452

98.178

0.544
  comFA/cflA Streptococcus pneumoniae D39

55.452

98.178

0.544
  comFA/cflA Streptococcus pneumoniae R6

55.452

98.178

0.544
  comFA/cflA Streptococcus mitis NCTC 12261

55.116

97.95

0.54
  comFA/cflA Streptococcus mitis SK321

54.186

97.95

0.531
  comFA Lactococcus lactis subsp. cremoris KW2

45.714

95.672

0.437
  comFA Latilactobacillus sakei subsp. sakei 23K

37.011

99.089

0.367

Multiple sequence alignment