Detailed information NTG1142875

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: DQN68_RS01380
• Genome accession: NZ_LS483522
• Coordinates: 251899..252969 (+)
• Length: 356 a.a.
• NCBI ID: WP_002992231.1
• Uniprot ID: A0A5S4TWS5
• Organism: Streptococcus pyogenes strain NCTC8224
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 246899..257969

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQN68_RS01365 (NCTC8224_00272)	amiA	247427..249397 (+)	1971	WP_002992224.1	peptide ABC transporter substrate-binding protein	Regulator
DQN68_RS01370 (NCTC8224_00273)	amiC	249462..250964 (+)	1503	WP_011017334.1	ABC transporter permease	Regulator
DQN68_RS01375 (NCTC8224_00274)	amiD	250964..251890 (+)	927	WP_002986004.1	oligopeptide ABC transporter permease OppC	Regulator
DQN68_RS01380 (NCTC8224_00275)	amiE	251899..252969 (+)	1071	WP_002992231.1	ABC transporter ATP-binding protein	Regulator
DQN68_RS01385 (NCTC8224_00276)	amiF	252962..253885 (+)	924	WP_002986000.1	ABC transporter ATP-binding protein	Regulator
DQN68_RS09690	-	253923..254012 (-)	90	WP_109821088.1	IS3 family transposase	-
DQN68_RS09425	-	254033..254212 (-)	180	WP_014635282.1	hypothetical protein	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39680.74 Da
• Isoelectric Point: 5.4774

>NTDB_id=1142875 DQN68_RS01380 WP_002992231.1 251899..252969(+) (amiE) [Streptococcus pyogenes strain NCTC8224]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSNAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=1142875 DQN68_RS01380 WP_002992231.1 251899..252969(+) (amiE) [Streptococcus pyogenes strain NCTC8224]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATCCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATCGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTAATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATCTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A5S4TWS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537