Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DQL17_RS03805 Genome accession   NZ_LS483496
Coordinates   740324..740863 (-) Length   179 a.a.
NCBI ID   WP_111688902.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC8455     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 735324..745863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL17_RS03775 (NCTC8455_00746) fdx 735727..736068 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  DQL17_RS03780 (NCTC8455_00747) iscX 736068..736262 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  DQL17_RS03785 (NCTC8455_00748) - 736313..736927 (-) 615 WP_005691752.1 YfgM family protein -
  DQL17_RS03790 (NCTC8455_00749) hisS 736945..738216 (-) 1272 WP_111688899.1 histidine--tRNA ligase -
  DQL17_RS03795 (NCTC8455_00750) ispG 738226..739332 (-) 1107 WP_111688900.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DQL17_RS03800 (NCTC8455_00751) - 739341..740252 (-) 912 WP_111688901.1 RodZ domain-containing protein -
  DQL17_RS03805 (NCTC8455_00752) pilF/pilF2 740324..740863 (-) 540 WP_111688902.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DQL17_RS03810 (NCTC8455_00753) - 740964..742115 (-) 1152 WP_162632306.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DQL17_RS03815 (NCTC8455_00754) - 742362..743240 (+) 879 WP_075985546.1 D-alanyl-D-alanine carboxypeptidase family protein -
  DQL17_RS03820 (NCTC8455_00755) - 743601..744482 (+) 882 WP_111688904.1 metal ABC transporter substrate-binding protein -
  DQL17_RS03825 (NCTC8455_00756) - 744482..745402 (+) 921 WP_050822774.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20519.23 Da        Isoelectric Point: 9.2107

>NTDB_id=1142730 DQL17_RS03805 WP_111688902.1 740324..740863(-) (pilF/pilF2) [Haemophilus influenzae strain NCTC8455]
MQTISKQLSAVILPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHNKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMNIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1142730 DQL17_RS03805 WP_111688902.1 740324..740863(-) (pilF/pilF2) [Haemophilus influenzae strain NCTC8455]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTACCTTTCATTTTTTCTGCCTGCGTTTCACAATCAGCATCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACAATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGAATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

94.972

100

0.95
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment