Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DQL22_RS00230 Genome accession   NZ_LS483485
Coordinates   47368..47922 (-) Length   184 a.a.
NCBI ID   WP_109842957.1    Uniprot ID   -
Organism   Aggregatibacter aphrophilus strain NCTC11096     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 42368..52922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL22_RS00205 (NCTC11096_00041) - 42530..42982 (-) 453 WP_111300658.1 methyltransferase family protein -
  DQL22_RS00210 (NCTC11096_00042) - 43038..43652 (-) 615 WP_109842955.1 YfgM family protein -
  DQL22_RS00215 (NCTC11096_00043) hisS 43663..44940 (-) 1278 WP_005701644.1 histidine--tRNA ligase -
  DQL22_RS00220 (NCTC11096_00044) ispG 44965..46068 (-) 1104 WP_005703458.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DQL22_RS00225 (NCTC11096_00045) - 46079..47149 (-) 1071 WP_032994995.1 helix-turn-helix domain-containing protein -
  DQL22_RS00230 (NCTC11096_00046) pilF/pilF2 47368..47922 (-) 555 WP_109842957.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DQL22_RS00235 (NCTC11096_00047) - 47944..49098 (-) 1155 WP_005701648.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DQL22_RS00240 (NCTC11096_00048) ansB 49396..50445 (+) 1050 WP_005701650.1 L-asparaginase 2 -
  DQL22_RS00245 (NCTC11096_00049) - 50609..51940 (+) 1332 WP_111300656.1 DUF4123 domain-containing protein -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20982.87 Da        Isoelectric Point: 7.2833

>NTDB_id=1142579 DQL22_RS00230 WP_109842957.1 47368..47922(-) (pilF/pilF2) [Aggregatibacter aphrophilus strain NCTC11096]
MVNIQFIFYRFVPFIFPFLFSACVVHSDAPDFDKQAAAKARVELALGYLQQQDGSQAKLNLDKALSYAPKYYLVHAALAY
FYQQQGDVEHAKQSYLTAIKLDDKQGDVFNNFGAFLCSQGEYQAAYEQFKQALKSPNYYHQADTYENLALCALSAKEQKI
YQENLAALEKIEPARAKKLTQFVK

Nucleotide


Download         Length: 555 bp        

>NTDB_id=1142579 DQL22_RS00230 WP_109842957.1 47368..47922(-) (pilF/pilF2) [Aggregatibacter aphrophilus strain NCTC11096]
ATGGTCAATATTCAGTTTATTTTTTACCGTTTTGTACCATTTATTTTTCCTTTTCTGTTTTCTGCCTGTGTTGTCCATTC
CGATGCTCCGGATTTTGATAAACAAGCGGCGGCAAAAGCCCGTGTGGAGTTGGCATTGGGCTATTTACAGCAGCAAGATG
GCAGCCAGGCCAAGTTGAATTTGGATAAAGCCCTTTCTTATGCCCCAAAATATTATTTGGTACATGCCGCGTTAGCCTAT
TTTTATCAGCAACAAGGGGATGTTGAACACGCCAAACAATCGTATTTAACGGCGATTAAACTGGATGATAAACAAGGGGA
TGTATTCAATAATTTCGGTGCGTTTCTTTGCTCACAAGGGGAATATCAGGCCGCGTATGAACAATTTAAACAGGCATTAA
AAAGCCCGAATTATTATCATCAAGCTGACACCTACGAAAACCTTGCACTGTGTGCGTTATCGGCAAAAGAGCAGAAAATT
TATCAAGAGAACCTTGCTGCTCTTGAAAAGATTGAACCGGCACGAGCCAAGAAATTAACTCAGTTCGTCAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(75-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

58.434

90.217

0.527
  pilF Glaesserella parasuis strain SC1401

42.703

100

0.429

Multiple sequence alignment